ArticleJournal of clinical microbiology1994
Typing of Candida krusei clinical isolates by restriction endonuclease analysis and hybridization with CkF1,2 DNA probe.
Article in Journal of clinical microbiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 32 citations in OpenAlex.
- Candida krusei (Pichia kudriavzevii) multilocus sequence typing and antifungal susceptibility profile in Cameroon.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Investigating Clinical Issues by Genotyping of Medically Important Fungi: Why and How?Clinical microbiology reviews · 2017Review
- Strain typing and determination of population structure of Candida krusei by multilocus sequence typing.Journal of clinical microbiology · 2007Article
- Comparison of PCR- and HinfI restriction endonuclease-based methods for typing of Candida krusei isolates.Journal of clinical microbiology · 2004Article
- The ins and outs of DNA fingerprinting the infectious fungi.Clinical microbiology reviews · 2000Review
- Molecular and epidemiological characterization of vaginal Saccharomyces cerevisiae isolates.Journal of clinical microbiology · 1999Article
- A monoclonal antibody specific to surface antigen on Candida krusei.Clinical and diagnostic laboratory immunology · 1998Article
- Identification of Candida species by randomly amplified polymorphic DNA fingerprinting of colony lysates.Journal of clinical microbiology · 1997Article
- Rapid identification and fingerprinting of Candida krusei by PCR-based amplification of the species-specific repetitive polymorphic sequence CKRS-1.Journal of clinical microbiology · 1997Article
- Comparison of rapid testing methods for enzyme production with the germ tube method for presumptive identification of Candida albicans.Journal of clinical microbiology · 1996Article
- Species-specific identification of Candida krusei by hybridization with the CkF1,2 DNA probe.Journal of clinical microbiology · 1996Article
- Strain variation among and antifungal susceptibilities of isolates of Candida krusei.Journal of clinical microbiology · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of restriction endonuclease analysis and Southern hybridization with our new CkF1,2 DNA probe, cold labeled with peroxidase, for the typing of Candida krusei isolates has been investigated. Fifty-five clinical samples isolated from forty-five patients hospitalized in eight centers, one environmental strain, and two reference strains were evaluated. Patterns were analyzed by a computer-assisted method and compared by numerical analysis. Clearer and less ambiguous patterns were obtained by restriction with endonuclease HinfI. It generated 9 to 14 (average, 11) well-separated fragments in the range of 6.5 to 2.0 kb. Both their numbers and sizes varied greatly among the strains studied. The CkF1,2 probe hybridized with one to seven fragments of HinfI patterns. A total of 48 distinct types were distinguished among the 58 strains studied. HinfI and CkF1,2 patterns showed similarities of less than 83 and 75% for unrelated strains and more than 91 and 100% for related strains, respectively. The methods showed 100% typeability, 98% reproducibility, and a discriminatory power of 1. C. krusei isolates from each patient were distinct, whether from one hospital or from different hospitals. Multiple isolates from the same patient were identical, both over time and at different anatomic sites. An endogenous origin is suggested for the colonizing and infecting isolates among the 45 patients. The CkF1,2 probe enhanced discrimination of the strains and provided a definitive comparison for strain identity. Genetic linkages between isolates were assessed at the subspecies level, and 12 clusters were delineated. A typing scheme is proposed for epidemiological studies of C. krusei.
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