ArticleThe Journal of cell biology1994
nup1 mutants exhibit pleiotropic defects in nuclear pore complex function.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Nuclear pore basket proteins are tethered to the nuclear envelope and can regulate membrane curvature.Developmental cell · 2015Article
- Review
- Structural characterization of altered nucleoporin Nup153 expression in human cells by thin-section electron microscopy.Nucleus (Austin, Tex.) · 2014Article
- Shaping the nucleus: factors and forces.Journal of cellular biochemistry · 2012Review
- Time-course proteome analysis reveals the dynamic response of Cryptococcus gattii cells to fluconazole.PloS one · 2012Article
- Article
- Changes in the nuclear envelope environment affect spindle pole body duplication in Saccharomyces cerevisiae.Genetics · 2010Article
- Members of the RSC chromatin-remodeling complex are required for maintaining proper nuclear envelope structure and pore complex localization.Molecular biology of the cell · 2010Article
- Using affinity chromatography to investigate novel protein-protein interactions in an undergraduate cell and molecular biology lab course.CBE life sciences education · 2009Article
- Mutations affecting spindle pole body and mitotic exit network function are synthetically lethal with a deletion of the nucleoporin NUP1 in S. cerevisiae.Current genetics · 2008Article
- Nuclear pore complex function in Saccharomyces cerevisiae is influenced by glycosylation of the transmembrane nucleoporin Pom152p.Genetics · 2005Article
- Novel nuclear herniations induced by nuclear localization of a viral protein.Journal of virology · 2004Article
- Chl1p, a DNA helicase-like protein in budding yeast, functions in sister-chromatid cohesion.Genetics · 2004Article
- The mRNA export machinery requires the novel Sac3p-Thp1p complex to dock at the nucleoplasmic entrance of the nuclear pores.The EMBO journal · 2002Article
- A link between the synthesis of nucleoporins and the biogenesis of the nuclear envelope.The Journal of cell biology · 2001Article
- Nup2p, a yeast nucleoporin, functions in bidirectional transport of importin alpha.Molecular and cellular biology · 2000Article
- Factors affecting nuclear export of the 60S ribosomal subunit in vivo.Molecular biology of the cell · 2000Article
- Nup124p is a nuclear pore factor of Schizosaccharomyces pombe that is important for nuclear import and activity of retrotransposon Tf1.Molecular and cellular biology · 1999Article
- Crm1p mediates regulated nuclear export of a yeast AP-1-like transcription factor.The EMBO journal · 1998Article
- A novel complex of membrane proteins required for formation of a spherical nucleus.The EMBO journal · 1998Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The NUP1 gene of Saccharomyces cerevisiae encodes one member of a family of nuclear pore complex proteins (nucleoporins) conserved from yeast to vertebrates. We have used mutational analysis to investigate the function of Nup1p. Deletion of either the amino- or carboxy-terminal domain confers a lethal phenotype, but partial truncations at either end affect growth to varying extents. Amino-terminal truncation causes mislocalization and degradation of the mutant protein, suggesting that this domain is required for targeting Nup1p to the nuclear pore complex. Carboxy-terminal mutants are stable but do not have wild-type function, and confer a temperature sensitive phenotype. Both import of nuclear proteins and export of poly(A) RNA are defective at the nonpermissive temperature. In addition, nup1 mutant cells become multinucleate at all temperatures, a phenotype suggestive of a defect in nuclear migration. Tubulin staining revealed that the mitotic spindle appears to be oriented randomly with respect to the bud, in spite of the presence of apparently normal cytoplasmic microtubules connecting one spindle pole body to the bud tip. EM analysis showed that the nuclear envelope forms long projections extending into the cytoplasm, which appear to have detached from the bulk of the nucleus. Our results suggest that Nup1p may be required to retain the structural integrity between the nuclear envelope and an underlying nuclear scaffold, and that this connection is required to allow reorientation of the nucleus in response to cytoskeletal forces.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.