ArticleThe Journal of cell biology1994
Identification of the plakoglobin-binding domain in desmoglein and its role in plaque assembly and intermediate filament anchorage.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 136 citations in OpenAlex.
- The molecular architecture of the desmosomal outer dense plaque by integrative structural modeling.bioRxiv : the preprint server for biology · 2024Article
- The intercalated disc: a unique organelle for electromechanical synchrony in cardiomyocytes.Physiological reviews · 2023Review
- Structure and regulation of desmosomes in intercalated discs: Lessons from epithelia.Journal of anatomy · 2023Review
- Understanding the molecular basis of cardiomyopathy.American journal of physiology. Heart and circulatory physiology · 2022Review
- The desmosome as a model for lipid raft driven membrane domain organization.Biochimica et biophysica acta. Biomembranes · 2020Review
- The Evolving Story of Autoantibodies in Pemphigus Vulgaris: Development of the "Super Compensation Hypothesis".Frontiers in medicine · 2018Review
- An autosomal recessive mutation of DSG4 causes monilethrix through the ER stress response.The Journal of investigative dermatology · 2015Article
- The expression of gingival epithelial junctions in response to subgingival biofilms.Virulence · 2015Article
- Cell adhesion in epidermal development and barrier formation.Current topics in developmental biology · 2015Review
- Different roles of cadherins in the assembly and structural integrity of the desmosome complex.Journal of cell science · 2014Article
- Mechanistic basis of desmosome-targeted diseases.Journal of molecular biology · 2013Review
- Desmoglein-1/Erbin interaction suppresses ERK activation to support epidermal differentiation.The Journal of clinical investigation · 2013Article
- Desmosomes: new perpetrators in tumour suppression.Nature reviews. Cancer · 2011Review
- Plakoglobin rescues adhesive defects induced by ectodomain truncation of the desmosomal cadherin desmoglein 1: implications for exfoliative toxin-mediated skin blistering.The American journal of pathology · 2010Article
- Novel missense mutations in exon 15 of desmoglein-2: role of the intracellular cadherin segment in arrhythmogenic right ventricular cardiomyopathy?Heart rhythm · 2010Article
- Interactions of plakoglobin and beta-catenin with desmosomal cadherins: basis of selective exclusion of alpha- and beta-catenin from desmosomes.The Journal of biological chemistry · 2009Article
- Discovering the molecular components of intercellular junctions--a historical view.Cold Spring Harbor perspectives in biology · 2009Review
- Loss of the desmosomal protein perp enhances the phenotypic effects of pemphigus vulgaris autoantibodies.The Journal of investigative dermatology · 2009Article
- Identification of a novel intermediate filament-linked N-cadherin/gamma-catenin complex involved in the establishment of the cytoarchitecture of differentiated lens fiber cells.Developmental biology · 2008Article
- The desmosome and pemphigus.Histochemistry and cell biology · 2008Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The carboxyterminal cytoplasmic portions (tails) of desmosomal cadherins of both the desmoglein (Dsg) and desmocollin type are integral components of the desmosomal plaque and are involved in desmosome assembly and the anchorage of intermediate-sized filaments. When additional Dsg tails were introduced by cDNA transfection into cultured human epithelial cells, in the form of chimeras with the aminoterminal membrane insertion domain of rat connexin32 (Co32), the resulting stably transfected cells showed a dominant-negative defect specific for desmosomal junctions: despite the continual presence of all desmosomal proteins, the endogenous desmosomes disappeared and the formation of Co32-Dsg chimeric gap junctions was inhibited. Using cell transfection in combination with immunoprecipitation techniques, we have examined a series of deletion mutants of the Dsg1 tail in Co32-Dsg chimeras. We show that upon removal of the last 262 amino acids the truncated Dsg tail still effects the binding of plakoglobin but not of detectable amounts of any catenin and induces the dominant-negative phenotype. However, further truncation or excision of the next 41 amino acids, which correspond to the highly conserved carboxyterminus of the C-domain in other cadherins, abolishes plakoglobin binding and allows desmosomes to reform. Therefore, we conclude that this short segment provides a plakoglobin-binding site and is important for plaque assembly and the specific anchorage of either actin filaments in adherens junctions or IFs in desmosomes.
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