ArticleThe Journal of cell biology1994
ACT3: a putative centractin homologue in S. cerevisiae is required for proper orientation of the mitotic spindle.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed.
- Molecular and functional basis for the scaffolding role of the p50/dynamitin subunit of the microtubule-associated dynactin complex.The Journal of biological chemistry · 2010Article
- Dynactin is required for coordinated bidirectional motility, but not for dynein membrane attachment.Molecular biology of the cell · 2007Article
- Single site alpha-tubulin mutation affects astral microtubules and nuclear positioning during anaphase in Saccharomyces cerevisiae: possible role for palmitoylation of alpha-tubulin.Molecular biology of the cell · 2001Article
- Bim1p/Yeb1p mediates the Kar9p-dependent cortical attachment of cytoplasmic microtubules.Molecular biology of the cell · 2000Article
- Microtubule interactions with the cell cortex causing nuclear movements in Saccharomyces cerevisiae.The Journal of cell biology · 2000Article
- Time-lapse video microscopy analysis reveals astral microtubule detachment in the yeast spindle pole mutant cnm67.Molecular biology of the cell · 2000Article
- Dynein intermediate chain mediated dynein-dynactin interaction is required for interphase microtubule organization and centrosome replication and separation in Dictyostelium.The Journal of cell biology · 1999Article
- Cytoplasmic dynein is required for distinct aspects of MTOC positioning, including centrosome separation, in the one cell stage Caenorhabditis elegans embryo.The Journal of cell biology · 1999Article
- The cortical localization of the microtubule orientation protein, Kar9p, is dependent upon actin and proteins required for polarization.The Journal of cell biology · 1999Article
- Characterization of the p22 subunit of dynactin reveals the localization of cytoplasmic dynein and dynactin to the midbody of dividing cells.The Journal of cell biology · 1998Article
- The kinesin-related proteins, Kip2p and Kip3p, function differently in nuclear migration in yeast.Molecular biology of the cell · 1998Article
- The yeast dynactin complex is involved in partitioning the mitotic spindle between mother and daughter cells during anaphase B.Molecular biology of the cell · 1998Article
- Evidence for a role of CLIP-170 in the establishment of metaphase chromosome alignment.The Journal of cell biology · 1998Article
- Saccharomyces cerevisiae cells with defective spindle pole body outer plaques accomplish nuclear migration via half-bridge-organized microtubules.Molecular biology of the cell · 1998Article
- Kar9p is a novel cortical protein required for cytoplasmic microtubule orientation in yeast.The Journal of cell biology · 1998Article
- Overexpression of the dynamitin (p50) subunit of the dynactin complex disrupts dynein-dependent maintenance of membrane organelle distribution.The Journal of cell biology · 1997Article
- Motoring to the finish: kinesin and dynein work together to orient the yeast mitotic spindle.The Journal of cell biology · 1997Review
- Deletion of nudC, a nuclear migration gene of Aspergillus nidulans, causes morphological and cell wall abnormalities and is lethal.Molecular biology of the cell · 1997Article
- Mutational analysis of Mdm1p function in nuclear and mitochondrial inheritance.The Journal of cell biology · 1997Article
- Microtubules orient the mitotic spindle in yeast through dynein-dependent interactions with the cell cortex.The Journal of cell biology · 1997Article
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2 authors.
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Abstract
As part of our ongoing efforts to understand the functional role of vertebrate centractins, we have identified a new member of the actin-related family of proteins in the yeast Saccharomyces cerevisiae using a PCR-based approach. Consistent with the current nomenclature for actin-related proteins in yeast, we propose to denote this locus ACT3. The primary amino acid sequence of Act3p is most similar to canine and human alpha-centractin (73% similarity/54% identity). The sequence of a genomic clone indicates ACT3 lies adjacent to and is transcribed convergently with respect to FUR1 on chromosome VIII. Molecular genetic analysis indicates ACT3 is represented by a single gene from which the corresponding mRNA is expressed at a low level compared to ACT1. Tetrad analysis of heterozygotes harboring a TRP1 replacement of the ACT3-coding region indicates ACT3 is nonessential for growth under normal conditions and at extremes of temperature and osmolarity. However, growth at 14 degrees C indicates a spindle orientation defect similar to phenotypes recently described for yeast harboring mutations in actin, tubulin, or cytoplasmic dynein. Taken together, our data suggest that ACT3 is the S. cerevisiae homologue of vertebrate centractins.
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