ArticleThe Journal of cell biology1994
Electric field-directed fibroblast locomotion involves cell surface molecular reorganization and is calcium independent.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
51 citing papers in PubMed, 1 synthesis or guideline pooled it, 177 citations in OpenAlex.
- Making Sense of Electrical Stimulation: A Meta-analysis for Wound Healing.Annals of biomedical engineering · 2024Pooled it
- Age-related changes in calcium ion influx and efflux capacity of human dermal fibroblasts.Cell communication and signaling : CCS · 2026Article
- Recent advancements in tissue dissociation techniques for cell manufacturing single-cell analysis and downstream processing.Stem cells translational medicine · 2025Review
- Electrical Stimulation of Cells: Drivers, Technology, and Effects.Chemical reviews · 2025Review
- The dynamics of chemoattractant receptors redistribution in the electrotaxis of 3T3 fibroblasts.Cell communication and signaling : CCS · 2025Article
- Cell shape and orientation control galvanotactic accuracy.Soft matter · 2024Article
- Effect of Electromagnetic Field on Proliferation and Migration of Fibroblasts and Keratinocytes: Implications in Wound Healing and Regeneration.Journal of biotechnology and biomedicine · 2024Article
- Bronchial Fibroblasts from Asthmatic Patients Display Impaired Responsiveness to Direct Current Electric Fields (dcEFs).Biomedicines · 2023Article
- Bioelectronic microfluidic wound healing: a platform for investigating direct current stimulation of injured cell collectives.Lab on a chip · 2023Article
- Advances in Biodegradable 3D Printed Scaffolds with Carbon-Based Nanomaterials for Bone Regeneration.Materials (Basel, Switzerland) · 2020Review
- Modeling the cornea in 3-dimensions: Current and future perspectives.Experimental eye research · 2020Review
- Mechanical Model for Durotactic Cell Migration.ACS biomaterials science & engineering · 2019Article
- Expression of integrins to control migration direction of electrotaxis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Electrical Stimulation Activates Fibroblasts through the Elevation of Intracellular Free CaBioMed research international · 2019Article
- Mechanisms of invasion and motility of high-grade gliomas in the brain.Molecular biology of the cell · 2018Review
- Reversing the direction of galvanotaxis with controlled increases in boundary layer viscosity.Physical biology · 2018Article
- Electrical stimulation structurally affects the tissues of the rectum and anus of nulliparous rats.Journal of anatomy · 2017Article
- Electrochemical Potential Gradient as a Quantitative in Vitro Test Platform for Cellular Oxidative Stress.Antioxidants (Basel, Switzerland) · 2016Article
- The Role of Kv1.2 Channel in Electrotaxis Cell Migration.Journal of cellular physiology · 2016Article
- Uniform electric field generation in circular multi-well culture plates using polymeric inserts.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Directional cellular locomotion is thought to involve localized intracellular calcium changes and the lateral transport of cell surface molecules. We have examined the roles of both calcium and cell surface glycoprotein redistribution in the directional migration of two murine fibroblastic cell lines, NIH 3T3 and SV101. These cell types exhibit persistent, cathode directed motility when exposed to direct current electric fields. Using time lapse phase contrast microscopy and image analysis, we have determined that electric field-directed locomotion in each cell type is a calcium independent process. Both exhibit cathode directed motility in the absence of extracellular calcium, and electric fields cause no detectable elevations or gradients of cytosolic free calcium. We find evidence suggesting that galvanotaxis in these cells involves the lateral redistribution of plasma membrane glycoproteins. Electric fields cause the lateral migration of plasma membrane concanavalin A receptors toward the cathode in both NIH 3T3 and SV101 fibroblasts. Exposure of directionally migrating cells to Con A inhibits the normal change of cell direction following a reversal of electric field polarity. Additionally, when cells are plated on Con A-coated substrata so that Con A receptors mediate cell-substratum adhesion, cathode-directed locomotion and a cathodal accumulation of Con A receptors are observed. Immunofluorescent labeling of the fibronectin receptor in NIH 3T3 fibroblasts suggests the recruitment of integrins from large clusters to form a more diffuse distribution toward the cathode in field-treated cells. Our results indicate that the mechanism of electric field directed locomotion in NIH 3T3 and SV101 fibroblasts involves the lateral redistribution of plasma membrane glycoproteins involved in cell-substratum adhesion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.