ArticleJournal of bacteriology1994
The Escherichia coli AlkB protein protects human cells against alkylation-induced toxicity.
Article in Journal of bacteriology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 70 citations in OpenAlex.
- Multi-substrate selectivity based on key loops and non-homologous domains: new insight into ALKBH family.Cellular and molecular life sciences : CMLS · 2021Review
- Programming of Cell Resistance to Genotoxic and Oxidative Stress.Biomedicines · 2018Review
- The AlkB Family of Fe(II)/α-Ketoglutarate-dependent Dioxygenases: Repairing Nucleic Acid Alkylation Damage and Beyond.The Journal of biological chemistry · 2015Review
- Highly Predictive Reprogramming of tRNA Modifications Is Linked to Selective Expression of Codon-Biased Genes.Chemical research in toxicology · 2015Article
- Nucleic acid oxidation in DNA damage repair and epigenetics.Chemical reviews · 2014Review
- Frameshift mutagenesis and microsatellite instability induced by human alkyladenine DNA glycosylase.Molecular cell · 2010Article
- Pediatric brain tumors: mutations of two dioxygenases (hABH2 and hABH3) that directly repair alkylation damage.Journal of neuro-oncology · 2009Article
- A non-heme iron-mediated chemical demethylation in DNA and RNA.Accounts of chemical research · 2009Article
- The complete genome sequence and comparative genome analysis of the high pathogenicity Yersinia enterocolitica strain 8081.PLoS genetics · 2006Article
- Methylating agents and DNA repair responses: Methylated bases and sources of strand breaks.Chemical research in toxicology · 2006Review
- Oxidative dealkylation DNA repair mediated by the mononuclear non-heme iron AlkB proteins.Journal of inorganic biochemistry · 2006Review
- Direct reversal of DNA alkylation damage.Chemical reviews · 2006Review
- Mutagenesis, genotoxicity, and repair of 1-methyladenine, 3-alkylcytosines, 1-methylguanine, and 3-methylthymine in alkB Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Reversal of DNA alkylation damage by two human dioxygenases.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Review
- The DNA-repair protein AlkB, EGL-9, and leprecan define new families of 2-oxoglutarate- and iron-dependent dioxygenases.Genome biology · 2001Article
- Regulatory responses of the adaptive response to alkylation damage: a simple regulon with complex regulatory features.Journal of bacteriology · 2000Article
- Defective processing of methylated single-stranded DNA by E. coli AlkB mutants.Genes & development · 2000Article
- Linkage map of Escherichia coli K-12, edition 10: the traditional map.Microbiology and molecular biology reviews : MMBR · 1998Review
- Generation of a strong mutator phenotype in yeast by imbalanced base excision repair.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Escherichia coli can ameliorate the toxic effects of alkylating agents either by preventing DNA alkylation or by repairing DNA alkylation damage. The alkylation-sensitive phenotype of E. coli alkB mutants marks the alkB pathway as an extremely effective defense mechanism against the cytotoxic effects of the SN2, but not the SN1, alkylating agents. Although it is clear that AlkB helps cells to better handle alkylated DNA, no DNA alkylation repair function could be assigned to the purified AlkB protein, suggesting that AlkB either acts as part of a complex or acts to regulate the expression of other genes whose products are directly responsible for alkylation resistance. However, here we present evidence that the provision of alkylation resistance is an intrinsic function of the AlkB protein per se. We expressed the E. coli AlkB protein in two human cell lines and found that it confers the same characteristic alkylation-resistant phenotype in this foreign environment as it does in E. coli. AlkB expression rendered human cells extremely resistant to cell killing by the SN2 but not the SN1 alkylating agents but did not affect the ability of dimethyl sulfate (an SN2 agent) to alkylate the genome. We infer that SN2 agents produce a class of DNA damage that is not efficiently produced by SN1 agents and that AlkB somehow prevents this damage from killing the cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.