ArticleJournal of bacteriology1994
Purification of Rhizobium leguminosarum HypB, a nickel-binding protein required for hydrogenase synthesis.
Article in Journal of bacteriology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 96 citations in OpenAlex.
- Structural investigations connect the disordered N-terminal extension of HypB to the activities of HypB and SlyD in E. coli.Protein science : a publication of the Protein Society · 2025Article
- Biosynthesis and assembly of hydrogenase [NiFe]-cofactor: recent advances and perspectives.Metallomics : integrated biometal science · 2025Review
- Proteomic and Mutant Analysis of Hydrogenase Maturation Protein GeneInternational journal of molecular sciences · 2023Article
- A Comparative Study on Nickel Binding to Hpn-like Polypeptides from TwoInternational journal of molecular sciences · 2021Article
- Nickel Metalloregulators and Chaperones.Inorganics · 2019Article
- A novel mechanism of "metal gel-shift" by histidine-rich Ni2+-binding Hpn protein from Helicobacter pylori strain SS1.PloS one · 2017Article
- Anaerobic Formate and Hydrogen Metabolism.EcoSal Plus · 2016Review
- Autoinhibition and signaling by the switch II motif in the G-protein chaperone of a radical B12 enzyme.The Journal of biological chemistry · 2013Article
- Specific metal recognition in nickel trafficking.Biochemistry · 2012Review
- Biochemical and structural studies on native and recombinant Glycine max UreG: a detailed characterization of a plant urease accessory protein.Plant molecular biology · 2012Article
- Structural basis for GTP-dependent dimerization of hydrogenase maturation factor HypB.PloS one · 2012Article
- Effects of metal on the biochemical properties of Helicobacter pylori HypB, a maturation factor of [NiFe]-hydrogenase and urease.Journal of bacteriology · 2011Article
- Binding of Ni2+ to a histidine- and glutamine-rich protein, Hpn-like.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2008Article
- Escherichia coli HypA is a zinc metalloprotein with a weak affinity for nickel.Journal of bacteriology · 2005Article
- HybF, a zinc-containing protein involved in NiFe hydrogenase maturation.Journal of bacteriology · 2004Article
- Identification and functional analysis of enzymes required for precorrin-2 dehydrogenation and metal ion insertion in the biosynthesis of sirohaem and cobalamin in Bacillus megaterium.The Biochemical journal · 2003Article
- Characterization of Helicobacter pylori nickel metabolism accessory proteins needed for maturation of both urease and hydrogenase.Journal of bacteriology · 2003Article
- Network of hydrogenase maturation in Escherichia coli: role of accessory proteins HypA and HybF.Journal of bacteriology · 2002Article
- Involvement of hyp gene products in maturation of the H(2)-sensing [NiFe] hydrogenase of Ralstonia eutropha.Journal of bacteriology · 2001Article
- Functional characterisation of urease accessory protein G (ureG) from potato.Plant molecular biology · 2001Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The products of the Rhizobium leguminosarum hyp gene cluster are necessary for synthesis of a functional uptake [NiFe] hydrogenase system in symbiosis with pea plants, and at least for HypB and HypF, a role in hydrogenase-specific nickel metabolism has been postulated (L. Rey, J. Murillo, Y. Hernando, E. Hidalgo, E. Cabrera, J. Imperial, and T. Ruiz-Argüeso, Mol. Microbiol. 8:471-481, 1993). The R. leguminosarum hypB gene product has been overexpressed in Escherichia coli and purified by immobilized nickel chelate affinity chromatography in a single step. The purified recombinant HypB protein was able to bind 3.9 +/- 0.1 Ni2+ ions per HypB monomer in solution. Co2+, Cu2+, and Zn2+ ions competed with Ni2+ with increasing efficiency. Monospecific HypB antibodies were raised and used to show that HypB is synthesized in R. leguminosarum microaerobic vegetative cells and pea bacteroids but not in R. leguminosarum aerobic cells. HypB protein synthesized by R. leguminosarum microaerobic vegetative cells could also be isolated by immobilized nickel chelate affinity chromatography. A histidine-rich region at the amino terminus of the protein (23-HGHHHH DGHHDHDHDHDHHRGDHEHDDHHH-54) is proposed to play a role in nickel binding, both in solution and in chelated form.
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