ArticleInfection and immunity1994
Response of Leishmania chagasi promastigotes to oxidant stress.
Article in Infection and immunity, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Isoenzyme characterization of Leishmania infantum toward checking the antioxidant activity of superoxide dismutase and glutathione peroxidase.BMC infectious diseases · 2024Article
- Access and utilization of host-derived iron by Leishmania parasites.Journal of biochemistry · 2023Review
- Global distribution of treatment resistance gene markers for leishmaniasis.Journal of clinical laboratory analysis · 2022Review
- Stimulation of metacyclogenesis inFrontiers in cellular and infection microbiology · 2022Article
- Ascorbate-Dependent Peroxidase (APX) from Leishmania amazonensis Is a Reactive Oxygen Species-Induced Essential Enzyme That Regulates Virulence.Infection and immunity · 2019Article
- ROS regulate differentiation of visceralizingParasitology open · 2018Article
- The iron-dependent mitochondrial superoxide dismutase SODA promotesThe Journal of biological chemistry · 2017Article
- Phosphorylation of Translation Initiation Factor 2-Alpha in Leishmania donovani under Stress Is Necessary for Parasite Survival.Molecular and cellular biology · 2017Article
- HSP70 of Leishmania amazonensis alters resistance to different stresses and mitochondrial bioenergetics.Memorias do Instituto Oswaldo Cruz · 2016Article
- Purification of a Fe-SOD excreted byParasite epidemiology and control · 2016Article
- Functional complementation of Leishmania (Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents.Memorias do Instituto Oswaldo Cruz · 2016Article
- Trypanosoma cruzi Needs a Signal Provided by Reactive Oxygen Species to Infect Macrophages.PLoS neglected tropical diseases · 2016Article
- Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection.Parasites & vectors · 2015Article
- IRONy OF FATE: role of iron-mediated ROS in Leishmania differentiation.Trends in parasitology · 2013Review
- Attenuation of Leishmania infantum chagasi metacyclic promastigotes by sterol depletion.Infection and immunity · 2013Article
- Iron uptake controls the generation of Leishmania infective forms through regulation of ROS levels.The Journal of experimental medicine · 2013Article
- Oxidative stress fuels Trypanosoma cruzi infection in mice.The Journal of clinical investigation · 2012Article
- Cloning, expression and dynamic simulation of TRYP6 from Leishmania major (MRHO/IR/75/ER).Molecular biology reports · 2011Article
- Resistance of Leishmania (Viannia) braziliensis to nitric oxide: correlation with antimony therapy and TNF-alpha production.BMC infectious diseases · 2010Article
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3 authors.
Funding
Abstract
At the onset of infection, Leishmania promastigotes are phagocytized by mammalian macrophages. They must survive despite exposure to toxic oxidants such as hydrogen peroxide (H2O2) and superoxide (.O2-) generated during phagocytosis. We investigated the effects of these oxidants on Leishmania chagasi promastigotes and promastigote mechanisms for oxidant resistance. According to spin trapping and electron paramagnetic resonance spectrometry, .O2- could be generated by exposure of promastigotes to the redox-cycling compound menadione. Incubation in either menadione or H2O2 caused a concentration-dependent loss of promastigote viability. However, incubation in sublethal concentrations of H2O2 or menadione caused a stress response in promastigotes. This oxidant-induced response was associated with an increase in the amount of heat shock protein hsp70. Induction of a stress response by exposure of promastigotes either to heat shock or to sublethal oxidants (H2O2 or menadione) caused promastigotes to become more resistant to H2O2 toxicity. Sublethal menadione also caused promastigotes to become more virulent in a BALB/c mouse model of leishmaniasis. We previously correlated H2O2 cytotoxicity for promastigotes with the formation of hydroxyl radical (.OH) from H2O2. However, according to electron paramagnetic resonance spectrometry, the increase in H2O2 resistance after exposure to sublethal oxidants was not associated with diminished generation (i.e., scavenging) of .OH. These data suggest that there is a cross-protective stress response that occurs after exposure of L. chagasi promastigotes to heat shock or to sublethal H2O2 or .O2-, exposures that also occur during natural infection. This response results in increased resistance to H2O2 toxicity and increased virulence for a mammalian host.
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