ArticleThe EMBO journal1994
The proteolysis-dependent metaphase to anaphase transition: calcium/calmodulin-dependent protein kinase II mediates onset of anaphase in extracts prepared from unfertilized Xenopus eggs.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 88 citations in OpenAlex.
- Maternal Proteins That Are Phosphoregulated upon Egg Activation Include Crucial Factors for Oogenesis, Egg Activation and Embryogenesis inG3 (Bethesda, Md.) · 2018Article
- Ion Channel Function During Oocyte Maturation and Fertilization.Frontiers in cell and developmental biology · 2018Review
- Calcium signaling and meiotic exit at fertilization in Xenopus egg.International journal of molecular sciences · 2014Review
- Molecular changes during egg activation.Current topics in developmental biology · 2013Review
- Regulation of store-operated Ca2+ entry during the cell cycle.Journal of cell science · 2010Article
- CaM kinase II initiates meiotic spindle depolymerization independently of APC/C activation.The Journal of cell biology · 2008Article
- Ca2+ homeostasis regulates Xenopus oocyte maturation.Biology of reproduction · 2008Article
- Regulation of Cdc2/cyclin B activation in Xenopus egg extracts via inhibitory phosphorylation of Cdc25C phosphatase by Ca(2+)/calmodulin-dependent protein [corrected] kinase II.Molecular biology of the cell · 2003Article
- The condensin complex is required for proper spindle assembly and chromosome segregation in Xenopus egg extracts.The Journal of cell biology · 2003Article
- A novel p21-activated kinase binds the actin and microtubule networks and induces microtubule stabilization.The Journal of cell biology · 2001Article
- Human 76p: A new member of the gamma-tubulin-associated protein family.The Journal of cell biology · 1999Article
- Fizzy is required for activation of the APC/cyclosome in Xenopus egg extracts.The EMBO journal · 1998Article
- MAPK inactivation is required for the G2 to M-phase transition of the first mitotic cell cycle.The EMBO journal · 1997Article
- A member of the Ste20/PAK family of protein kinases is involved in both arrest of Xenopus oocytes at G2/prophase of the first meiotic cell cycle and in prevention of apoptosis.The EMBO journal · 1997Article
- The Drosophila gene abnormal spindle encodes a novel microtubule-associated protein that associates with the polar regions of the mitotic spindle.The Journal of cell biology · 1997Article
- The 'destruction box' of cyclin A allows B-type cyclins to be ubiquitinated, but not efficiently destroyed.The EMBO journal · 1996Article
- Phragmoplastin, a dynamin-like protein associated with cell plate formation in plants.The EMBO journal · 1996Article
- Exit from mitosis is regulated by Drosophila fizzy and the sequential destruction of cyclins A, B and B3.The EMBO journal · 1995Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been shown, using spindles assembled in vitro in extracts containing CSF (the cytostatic factor responsible for arresting unfertilized vertebrate eggs at metaphase), that onset of anaphase requires Ca(2+)-dependent activation of the ubiquitin-dependent proteolytic pathway that destroys both mitotic cyclins and an unknown protein responsible for metaphase arrest (Holloway et al., 1993, Cell, 73, 1382-1402). We showed recently that Ca2+/calmodulin-dependent protein kinase II (CaM KII) activates the ubiquitin-dependent cyclin degradation pathway in CSF extracts (Lorca et al., 1993, Nature, 366, 270-273), but did not investigate its possible effect on sister chromatid segregation. In this work we identify CaM KII as the only target of Ca2+ in inducing anaphase in CSF extracts, and further show that transition to anaphase does not require the direct phosphorylation of metaphase spindle components by CaM KII. A possible interpretation of the above results could have been that the ubiquitin-dependent degradation pathway is required for onset of anaphase only when spindles are clamped at metaphase due to CSF activity, and not in the regular cell cycle that occurs in the absence of CSF activity. We ruled out this possibility by showing that competitive inhibition of the ubiquitin-dependent degradation pathway still prevents the onset of anaphase in cycling extracts that lack CSF and do not require Ca2+ for sister chromatid separation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.