Evidence map›Paper›PMID 7920258›Full record

ArticleProtein science : a publication of the Protein Society1994

Expression, purification, and characterization of thymidylate synthase from Lactococcus lactis.

P J Greene, P L Yu, J Zhao, C A Schiffer, D Santi

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein science : a publication of the Protein Society, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 58% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

P J GreeneDepartment of Biochemistry, University of California, San Francisco 94143.
P L Yu
J Zhao
C A Schiffer
D Santi
University of California, San Francisco · US

Funding

STRUCTURE/FUNCTION OF PROTEINS AT MOLECULAR LEVELR01GM024485 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI STROUD, ROBERT M · 1985 to 2024
$7.5M
Molecular Basis for Transmembrane Conduction & SignalingR37GM024485 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI STROUD, ROBERT M · 2007 to 2016
$4.7M
THYMIDYLATE SYNTHETASE AND RELATED ENZYMESR01CA014394 · NCI · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · PI STROUD, ROBERT M · 1985 to 2001
$708k
THYMIDYLATE SYNTHETASE AND RELATED ENZYMESR37CA014394 · NCI · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · PI SANTI, DANIEL V. · 1989 to 1997
–
NCI NIH HHS CA 14394NIGMS NIH HHS GM 24485
6 · The paper itself

Abstract

The thymidylate synthase (TS) gene from Lactococcus lactis has been highly expressed in Escherichia coli. The TS protein was purified by sequential chromatography on Q-Sepharose and phenyl-Sepharose. Six grams of cell pellet yielded 140 mg of homogeneous TS. TS is a highly conserved enzyme, and several of the conserved amino acid residues that have been implicated in catalytic function are altered in L. lactis TS. By use of a 3-dimensional homology model, we have predicted covariant changes that might compensate for these differences. With the large amounts of L. lactis TS now available, studies can be pursued to understand the structure-function relationships of this enzyme compared to other TSs and to confirm the presumed roles of the compensatory changes predicted in the homology model.

Indexed as

Gene ExpressionAmino Acid SequenceBinding SitesCrystallizationEscherichia coliHydrogen-Ion ConcentrationLactococcus lactisModels, MolecularMolecular Sequence DataMolecular StructureOsmolar ConcentrationRecombinant ProteinsStructure-Activity RelationshipThymidylate SynthaseRecombinant ProteinsThymidylate Synthase

Identifiers

PMID7920258
PMCPMC2142890
OpenAlexW2021980846

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.