ArticleMolecular biology of the cell1994
Bundling of microtubules in transfected cells does not involve an autonomous dimerization site on the MAP2 molecule.
Article in Molecular biology of the cell, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 27 citations in OpenAlex.
- Conserved Lysine Acetylation within the Microtubule-Binding Domain Regulates MAP2/Tau Family Members.PloS one · 2016Article
- Mechanical aspects of microtubule bundling in taxane-treated circulating tumor cells.Biophysical journal · 2014Article
- Developmental but not adult cannabinoid treatments persistently alter axonal and dendritic morphology within brain regions important for zebra finch vocal learning.Brain research · 2014Article
- The dual-specificity phosphatase CDC14B bundles and stabilizes microtubules.Molecular and cellular biology · 2005Article
- The MAP2/Tau family of microtubule-associated proteins.Genome biology · 2005Review
- Mitotic impairment by doublecortin is diminished by doublecortin mutations found in patients.Neurogenetics · 2004Article
- The role of microtubule-associated protein 2c in the reorganization of microtubules and lamellipodia during neurite initiation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Herpes simplex virus tegument protein VP22 contains overlapping domains for cytoplasmic localization, microtubule interaction, and chromatin binding.Journal of virology · 2002Article
- Phosphorylation-dependent localization of microtubule-associated protein MAP2c to the actin cytoskeleton.Molecular biology of the cell · 2000Article
- Cytoplasm-to-nucleus translocation of a herpesvirus tegument protein during cell division.Journal of virology · 2000Article
- Herpes simplex virus type 1 tegument protein VP22 induces the stabilization and hyperacetylation of microtubules.Journal of virology · 1998Article
- Transgenic expression of embryonic MAP2 in adult mouse brain: implications for neuronal polarization.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
- Analysis of MAP 4 function in living cells using green fluorescent protein (GFP) chimeras.The Journal of cell biology · 1995Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have searched for putative dimerization sites in microtubule-associated protein 2 (MAP2) that may be involved in the bundling of microtubules. An overlapping series of fragments of the embryonic form MAP2c were created and immunologically "tagged" with an 11 amino acid sequence from human c-myc. Nonneuronal cells were transfected simultaneously with one of these myc-tagged fragments and with full-length native MAP2c. Immunolabeling with site-specific antibodies allowed the two transgene products to be located independently within the cytoplasm of a single double-transfected cell. All transfected cells contained bundled microtubules to which the full-length native MAP2 was bound. The distribution of the tagged MAP2 fragment relative to these MAP2-induced bundles was determined by the anti-myc staining. None of the fragments tested, representing all of the MAP2c sequence in overlapping pieces, were associated with MAP2-induced microtubule bundles. These results suggest that MAP2-induced bundle formation in cells does not involve an autonomous dimerization site within the MAP2 sequence.
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