ArticleBiophysical journal1994
Testing for microscopic reversibility in the gating of maxi K+ channels using two-dimensional dwell-time distributions.
Article in Biophysical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Markov models and long-term memory in ion channels: A contradiction in terms?Biophysical journal · 2025Article
- A deep learning approach to real-time Markov modeling of ion channel gating.Communications chemistry · 2024Article
- Microscopic mechanism of PIEZO1 activation by pressure-induced membrane stretch.The Journal of general physiology · 2023Article
- Hypothesized diprotomeric enzyme complex supported by stochastic modelling of palytoxin-induced Na/K pump channels.Royal Society open science · 2018Article
- Estimating kinetic mechanisms with prior knowledge I: Linear parameter constraints.The Journal of general physiology · 2018Article
- Elucidation of molecular kinetic schemes from macroscopic traces using system identification.PLoS computational biology · 2017Article
- Quantifying the cooperative subunit action in a multimeric membrane receptor.Scientific reports · 2016Article
- Single-channel kinetics of BK (Slo1) channels.Frontiers in physiology · 2014Review
- Transduction of voltage and Ca2+ signals by Slo1 BK channels.Physiology (Bethesda, Md.) · 2013Review
- Coupling and cooperativity in voltage activation of a limited-state BK channel gating in saturating Ca2+.The Journal of general physiology · 2010Article
- Utilizing the information content in two-state trajectories.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Intracellular Mg2+ influences both open and closed times of a native Ca2+-activated BK channel in cultured human renal proximal tubule cells.The Journal of membrane biology · 2005Article
- Gating reaction mechanisms for NMDA receptor channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Using independent open-to-closed transitions to simplify aggregated Markov models of ion channel gating kinetics.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Maximum likelihood estimation of ion channel kinetics from macroscopic currents.Biophysical journal · 2005Article
- How to impose microscopic reversibility in complex reaction mechanisms.Biophysical journal · 2004Article
- Calcium regulation of single ryanodine receptor channel gating analyzed using HMM/MCMC statistical methods.The Journal of general physiology · 2004Article
- Oxidative regulation of large conductance calcium-activated potassium channels.The Journal of general physiology · 2001Article
- The effects of non-identifiability on testing for detailed balance in aggregated Markov models for ion-channel gating.Biophysical journal · 2000Article
- Voltage and Ca2+ activation of single large-conductance Ca2+-activated K+ channels described by a two-tiered allosteric gating mechanism.The Journal of general physiology · 2000Article
Corrections and comments
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2 authors.
Funding
Abstract
An assumption usually made when developing kinetic models for the gating of ion channels is that the transitions among the various states involved in the gating obey microscopic reversibility. If this assumption is incorrect, then the models and estimated rate constants made with the assumption would be in error. This paper examines whether the gating of a large conductance Ca-activated K+ channel in skeletal muscle is consistent with microscopic reversibility. If microscopic reversibility is obeyed, then the number of forward and backward transitions per unit time for each individual reaction step will, on average, be identical and, consequently, the gating must show time reversibility. To look for time reversibility, two-dimensional dwell-time distributions of the durations of open and closed intervals were obtained from single-channel current records analyzed in the forward and in the backward directions. Two-dimensional dwell-time distributions of pairs of open intervals and of pairs of closed intervals were also analyzed to extend the resolution of the method to special circumstances in which intervals from different closed (or open) states might have similar durations. No significant differences were observed between the forward and backward analysis of the two-dimensional dwell-time distributions, suggesting time reversibility. Thus, we find no evidence to indicate that the gating of the maxi K+ channel violates microscopic reversibility.
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