ArticleJournal of bacteriology1994
Analysis of the sodium dodecyl sulfate-stable peptidoglycan autolysins of select gram-negative pathogens by using renaturing polyacrylamide gel electrophoresis.
Article in Journal of bacteriology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 75 citations in OpenAlex.
- Measure of Peptidoglycan Degradation Activity.Methods in molecular biology (Clifton, N.J.) · 2024Article
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- Proximate and ultimate causes of the bactericidal action of antibiotics.Nature reviews. Microbiology · 2021Review
- Uncovering the activities, biological roles, and regulation of bacterial cell wall hydrolases and tailoring enzymes.The Journal of biological chemistry · 2020Review
- False positives in using the zymogram assay for identification of peptidoglycan hydrolases.Analytical biochemistry · 2018Article
- Neisseria gonorrhoeae virulence factor NG1686 is a bifunctional M23B family metallopeptidase that influences resistance to hydrogen peroxide and colony morphology.The Journal of biological chemistry · 2012Article
- Characterization and functional analysis of atl, a novel gene encoding autolysin in Streptococcus suis.Journal of bacteriology · 2012Article
- Peptidoglycan hydrolases of Escherichia coli.Microbiology and molecular biology reviews : MMBR · 2011Review
- Regulation of biofilm components in Salmonella enterica serovar Typhimurium by lytic transglycosylases involved in cell wall turnover.Journal of bacteriology · 2011Article
- The C-terminal domain of Escherichia coli YfhD functions as a lytic transglycosylase.The Journal of biological chemistry · 2008Article
- The Inactivation of a New Peptidoglycan Hydrolase Pmp23 Leads to Abnormal Septum Formation in Streptococcus pneumoniae.The open microbiology journal · 2008Article
- AtlA functions as a peptidoglycan lytic transglycosylase in the Neisseria gonorrhoeae type IV secretion system.Journal of bacteriology · 2007Article
- The entry mechanism of membrane-containing phage Bam35 infecting Bacillus thuringiensis.Journal of bacteriology · 2006Article
- Peptidoglycan O acetylation and autolysin profile of Enterococcus faecalis in the viable but nonculturable state.Journal of bacteriology · 2006Article
- Penetration of membrane-containing double-stranded-DNA bacteriophage PM2 into Pseudoalteromonas hosts.Journal of bacteriology · 2004Article
- The lytic enzyme of bacteriophage PRD1 is associated with the viral membrane.Journal of bacteriology · 2002Article
- Structures of gram-negative cell walls and their derived membrane vesicles.Journal of bacteriology · 1999Review
- Gram-negative bacteria produce membrane vesicles which are capable of killing other bacteria.Journal of bacteriology · 1998Article
- Membrane-bound lytic endotransglycosylase in Escherichia coli.Journal of bacteriology · 1998Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For the first time, peptidoglycan autolysins from cellular fractions derived from sonicated cultures of Pseudomonas aeruginosa PAO1, Escherichia coli W7, Klebsiella pneumoniae CWK2, and Proteus mirabilis 19 were detected and partially characterized by zymogram analysis. Purified murein sacculi from P. aeruginosa PAO1 were incorporated into a sodium dodecyl sulfate (SDS)-polyacrylamide gel at a concentration of 0.05% (wt/vol) to serve as a substrate for the separated autolysins. At least 11 autolysin bands of various intensities with M(r)s ranging between 17,000 and 122,000 were detected in each of the homogenated cultures. Some of the autolysins of the four bacteria had similar M(r)s. The zymogram analysis was used to show that a number of the autolysins from E. coli were inhibited by the heavy metals Hg2+ and Cu2+, at 1 and 10 mM, respectively, high ionic strengths, and reagents known to affect the packing of lipopolysaccharides. The activity of an autolysin with an M(r) of 65,000 was also impaired by penicillin G, whereas it was enhanced by gentamicin. A preliminary screen to determine the relationship between penicillin-binding proteins (PBPs) and autolysins was carried out by using a dual assay in which radiolabelled penicillin V bands were visualized on an autolysin zymogram. Radiolabelled bands corresponding to PBPs 3, 4, 5, and 6 from E. coli and P. aeruginosa; PBPs 3, 4, and 6 from Proteus mirabilis; and PBP 6 from K. pneumoniae degraded the murein sacculi in the gels and were presumed to have autolytic activity, although the possibility of two distinct enzymes, each with one of the activities, comigrating in the SDS-polyacrylamide gels could not be excluded. Some radiolabelled bands possessed an Mr of <34,000 and coincided with similar low-Mr autolysin bands.
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