ArticleProceedings of the National Academy of Sciences of the United States of America1994
"RFLP subtraction": a method for making libraries of polymorphic markers.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Modern Plant Breeding Techniques in Crop Improvement and Genetic Diversity: From Molecular Markers and Gene Editing to Artificial Intelligence-A Critical Review.Plants (Basel, Switzerland) · 2024Review
- An Ac-like transposable element family with transcriptionally active Y-linked copies in the white campion, Silene latifolia.Genetics · 2003Article
- Screening of gene-associated polymorphisms by use of in-gel competitive reassociation and EST (cDNA) array hybridization.Genome research · 2003Article
- NotI subtraction and NotI-specific microarrays to detect copy number and methylation changes in whole genomes.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Diversity arrays: a solid state technology for sequence information independent genotyping.Nucleic acids research · 2001Article
- Cloning of polymorphisms (COP): enrichment of polymorphic sequences from complex genomes.Nucleic acids research · 2000Article
- Subtracted, unique-sequence, in situ hybridization: experimental and diagnostic applications.The American journal of pathology · 1998Article
- Positional cloning without a genome map: using 'Targeted RFLP Subtraction' to isolate dense markers tightly linked to the regA locus of Volvox carteri.Nucleic acids research · 1998Article
- Ligation-mediated PCR amplification of specific fragments from a class-II restriction endonuclease total digest.Nucleic acids research · 1997Article
- Cytological and molecular characterization of oat x maize partial hybrids.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 1996Article
- From mutation mapping to phenotype cloning.Proceedings of the National Academy of Sciences of the United States of America · 1995Review
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Authors and funding
3 authors.
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Abstract
We developed a method, "RFLP subtraction," that isolates large numbers of unique sequence restriction fragment length polymorphisms (RFLPs) in a single experiment. The technique purifies small restriction fragments from one genome containing sequences that reside on large fragments in a related genome. We first isolate samples containing the small restriction fragments from two polymorphic strains. Subtractive hybridization then removes the fragments that are present in both samples. The remaining sequences are RFLPs: they occur on small fragments in one strain but not in the other. Here we use RFLP subtraction to make a library of hundreds of unique sequence RFLPs from two inbred mouse strains. We analyze and map a subset of the RFLPs and show that the genetic linkage of these markers can be rapidly determined by an efficient dot blot mapping technique. Several other potential applications of RFLP subtraction, including isolating region specific markers, are discussed.
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