ArticleProceedings of the National Academy of Sciences of the United States of America1994
Inhibition of retinoic acid-induced activation of 3' human HOXB genes by antisense oligonucleotides affects sequential activation of genes located upstream in the four HOX clusters.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 61 citations in OpenAlex.
- Homeobox A10 in gastrointestinal malignancies: unraveling metastatic mechanisms and novel therapeutic opportunities.Cancer metastasis reviews · 2026Review
- Unlocking the HOX: Homeobox Genes as Regulators of Hematopoietic Development.International journal of molecular sciences · 2026Review
- Antifibrotic factor KLF4 is repressed by the miR-10/TFAP2A/TBX5 axis in dermal fibroblasts: insights from twins discordant for systemic sclerosis.Annals of the rheumatic diseases · 2022Article
- The expression ofAnnals of translational medicine · 2022Article
- HOXC10 upregulation confers resistance to chemoradiotherapy in ESCC tumor cells and predicts poor prognosis.Oncogene · 2020Article
- A Tribute to Lewis Wolpert and His Ideas on the 50th Anniversary of the Publication of His Paper 'Positional Information and the Spatial Pattern of Differentiation'. Evidence for a Timing Mechanism for Setting Up the Vertebrate Anterior-Posterior (A-P) Axis.International journal of molecular sciences · 2020Review
- HOXD9 promotes the growth, invasion and metastasis of gastric cancer cells by transcriptional activation of RUFY3.Journal of experimental & clinical cancer research : CR · 2019Article
- What are the roles of retinoids, other morphogens, and Hox genes in setting up the vertebrate body axis?Genesis (New York, N.Y. : 2000) · 2019Review
- Vertebrate hox temporal collinearity: does it exist and what is it's function?Cell cycle (Georgetown, Tex.) · 2019Review
- Some Questions and Answers About the Role ofFrontiers in cell and developmental biology · 2019Article
- Two Tier Hox Collinearity Mediates Vertebrate Axial Patterning.Frontiers in cell and developmental biology · 2018Article
- Collinear Hox-Hox interactions are involved in patterning the vertebrate anteroposterior (A-P) axis.PloS one · 2017Article
- Article
- Histone methylases MLL1 and MLL3 coordinate with estrogen receptors in estrogen-mediated HOXB9 expression.Biochemistry · 2011Article
- Epigenetic regulations in hematopoietic Hox code.Oncogene · 2011Review
- Quantitative proteome analysis of pluripotent cells by iTRAQ mass tagging reveals post-transcriptional regulation of proteins required for ES cell self-renewal.Molecular & cellular proteomics : MCP · 2010Article
- Suppression of embryonic lung branching morphogenesis by antisense oligonucleotides against HOM/C homeobox factors.In vitro cellular & developmental biology. Animal · 2010Article
- Hox genes and their candidate downstream targets in the developing central nervous system.Cellular and molecular neurobiology · 2005Review
- Hematopoietic expression of HOXB4 is regulated in normal and leukemic stem cells through transcriptional activation of the HOXB4 promoter by upstream stimulating factor (USF)-1 and USF-2.The Journal of experimental medicine · 2000Article
- Induction of the angiogenic phenotype by Hox D3.The Journal of cell biology · 1997Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Most homeobox genes belonging to the Hox family are sequentially activated in embryonal carcinoma cells upon treatment with retinoic acid. Genes located at the 3' end of each one of the four Hox clusters are activated first, whereas upstream Hox genes are activated progressively later. This activation has been extensively studied for human HOX genes in the NT2/D1 cell line and shown to take place at the transcriptional level. To understand the molecular mechanisms of sequential HOX gene activation in these cells, we tried to modulate the expression of 3' HOX genes through the use of antisense oligonucleotides added to the culture medium. We chose the HOXB locus. A 5- to 15-fold reduction of the expression of HOXB1 and HOXB3 was sufficient to produce a significant inhibition of the activation of the upstream HOXB genes, as well as of their paralogs in the HOXA, HOXC, and HOXD clusters. Conversely, no effect was detectable on downstream HOX genes. The extent of this inhibition increased for progressively more-5' genes. The stability of the corresponding mRNAs appeared to be unaffected, supporting the idea that the observed effect might be mediated at the transcriptional level. These data suggest a cascade model of progressive activation of Hox genes, with a 3'-to-5' polarity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.