ArticleProceedings of the National Academy of Sciences of the United States of America1994
Electroporation-mediated replacement of a positively and negatively selectable beta-tubulin gene in Tetrahymena thermophila.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
52 citing papers in PubMed, 97 citations in OpenAlex.
- Global and local functions of the Fused kinase ortholog CdaH in intracellular patterning in Tetrahymena.Journal of cell science · 2024Article
- The tubulin database: Linking mutations, modifications, ligands and local interactions.PloS one · 2023Article
- Cfap91-Dependent Stability of the RS2 and RS3 Base Proteins and Adjacent Inner Dynein Arms inCells · 2022Article
- Mutual antagonism between Hippo signaling and cyclin E drives intracellular pattern formation.The Journal of cell biology · 2020Article
- Article
- LF4/MOK and a CDK-related kinase regulate the number and length of cilia in Tetrahymena.PLoS genetics · 2019Article
- Two Antagonistic Hippo Signaling Circuits Set the Division Plane at the Medial Position in the CiliateGenetics · 2019Article
- The CSC proteins FAP61 and FAP251 build the basal substructures of radial spoke 3 in cilia.Molecular biology of the cell · 2015Article
- A Tetrahymena Hsp90 co-chaperone promotes siRNA loading by ATP-dependent and ATP-independent mechanisms.The EMBO journal · 2015Article
- SUMOylation is developmentally regulated and required for cell pairing during conjugation in Tetrahymena thermophila.Eukaryotic cell · 2015Article
- DisAp-dependent striated fiber elongation is required to organize ciliary arrays.The Journal of cell biology · 2014Article
- Discovery and functional evaluation of ciliary proteins in Tetrahymena thermophila.Methods in enzymology · 2013Article
- Tetrahymena in the laboratory: strain resources, methods for culture, maintenance, and storage.Methods in cell biology · 2012Article
- Whole-cell biosensors for detection of heavy metal ions in environmental samples based on metallothionein promoters from Tetrahymena thermophila.Microbial biotechnology · 2011Article
- The two domains of centrin have distinct basal body functions in Tetrahymena.Molecular biology of the cell · 2011Article
- Expression, secretion and surface display of a human alkaline phosphatase by the ciliate Tetrahymena thermophila.BMC biotechnology · 2011Article
- α-Tubulin mutations alter oryzalin affinity and microtubule assembly properties to confer dinitroaniline resistance.Eukaryotic cell · 2010Article
- Mutation accumulation in Tetrahymena.BMC evolutionary biology · 2010Article
- Sequence, biogenesis, and function of diverse small RNA classes bound to the Piwi family proteins of Tetrahymena thermophila.Genes & development · 2009Article
- A single RNA-dependent RNA polymerase assembles with mutually exclusive nucleotidyl transferase subunits to direct different pathways of small RNA biogenesis.RNA (New York, N.Y.) · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Replacement of lysine-350 by methionine in the beta-tubulin gene of Chlamydomonas confers resistance to microtubule-depolymerizing drugs and increased sensitivity to the microtubule-stabilizing drug taxol. This mutation was created in cloned BTU1, one of two coexpressed beta-tublin genes of Tetrahymena thermophila. When introduced by electroporation, the mutated gene transformed Tetrahymena exclusively by gene replacement at the homologous locus. Taxol-sensitive transformants could be retransformed with a wild-type gene and selection for taxol resistance. Analyses of phenotypic assortment and of the mRNA in transformed cells suggest that complete replacement of the BTU1 gene in the polyploid macronucleus can be obtained. These studies demonstrate the utility of this marker for studying tublin gene function and show that electroporation allows facile gene replacement in Tetrahymena.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.