ArticleNucleic acids research1993
Isolation and characterization of target sequences of the chicken CdxA homeobox gene.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 119 citations in OpenAlex.
- CDX2 regulates ACE expression in blood development and leukemia cells.Blood advances · 2021Article
- A Core Response to the CDX2 Homeoprotein During Development and in Pathologies.Frontiers in genetics · 2021Article
- A Novel Heat Shock Element (HSE) inFrontiers in cellular and infection microbiology · 2017Article
- Extending the functions of the homeotic transcription factor Cdx2 in the digestive system through nontranscriptional activities.World journal of gastroenterology · 2015Review
- Genetic variants of the HSD11B1 gene promoter may be protective against polycystic ovary syndrome.Molecular biology reports · 2014Article
- The association of single nucleotide polymorphisms in the calcitonin gene with primary osteoarthritis of the knee in Mexican mestizo population.Rheumatology international · 2013Article
- CDX1 confers intestinal phenotype on gastric epithelial cells via induction of stemness-associated reprogramming factors SALL4 and KLF5.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Functional characterization of genetic polymorphisms identified in the promoter region of the bovine PEPS gene.DNA and cell biology · 2012Article
- Elongation factor 1 alpha1 and genes associated with Usher syndromes are downstream targets of GBX2.PloS one · 2012Article
- Reg IV is a direct target of intestinal transcriptional factor CDX2 in gastric cancer.PloS one · 2012Article
- Reactivation of transgene expression by alleviating CpG methylation of the Rous sarcoma virus promoter in transgenic quail cells.Molecular biotechnology · 2011Article
- Article
- CDX2 regulates multidrug resistance 1 gene expression in malignant intestinal epithelium.Cancer research · 2010Article
- Polymorphism in the flanking regions of the PbGP43 gene from the human pathogen Paracoccidioides brasiliensis: search for protein binding sequences and poly(A) cleavage sites.BMC microbiology · 2009Article
- AtTRP1 encodes a novel TPR protein that interacts with the ethylene receptor ERS1 and modulates development in Arabidopsis.Journal of experimental botany · 2009Article
- Molecular characterization of the ghrelin and ghrelin receptor genes and effects on fat deposition in chicken and duck.Journal of biomedicine & biotechnology · 2009Article
- A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system.Nucleic acids research · 2008Article
- Cdx protein interaction with Hoxa5 regulatory sequences contributes to Hoxa5 regional expression along the axial skeleton.Molecular and cellular biology · 2005Article
- A negative regulatory element (base pairs -204 to -177) of the EICP0 promoter of equine herpesvirus 1 abolishes the EICP0 protein's trans-activation of its own promoter.Journal of virology · 2004Article
- Ectopic expression of the homeobox gene Cdx2 is the transforming event in a mouse model of t(12;13)(p13;q12) acute myeloid leukemia.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The DNA binding specificity of the chicken homeodomain protein CDXA was studied. Using a CDXA-glutathione-S-transferase fusion protein, DNA fragments containing the binding site for this protein were isolated. The sources of DNA were oligonucleotides with random sequence and chicken genomic DNA. The DNA fragments isolated were sequenced and tested in DNA binding assays. Sequencing revealed that most DNA fragments are AT rich which is a common feature of homeodomain binding sites. By electrophoretic mobility shift assays it was shown that the different target sequences isolated bind to the CDXA protein with different affinities. The specific sequences bound by the CDXA protein in the genomic fragments isolated, were determined by DNase I footprinting. From the footprinted sequences, the CDXA consensus binding site was determined. The CDXA protein binds the consensus sequence A, A/T, T, A/T, A, T, A/G. The CAUDAL binding site in the ftz promoter is also included in this consensus sequence. When tested, some of the genomic target sequences were capable of enhancing the transcriptional activity of reporter plasmids when introduced into CDXA expressing cells. This study determined the DNA sequence specificity of the CDXA protein and it also shows that this protein can further activate transcription in cells in culture.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.