ArticleProceedings of the National Academy of Sciences of the United States of America1994
Drug-selected coexpression of human glucocerebrosidase and P-glycoprotein using a bicistronic vector.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 87 citations in OpenAlex.
- The multiple facets of drug resistance: one history, different approaches.Journal of experimental & clinical cancer research : CR · 2014Review
- Article
- Human cytidine deaminase as an ex vivo drug selectable marker in gene-modified primary bone marrow stromal cells.Gene therapy · 2002Article
- Drug selection with paclitaxel restores expression of linked IL-2 receptor gamma -chain and multidrug resistance (MDR1) transgenes in canine bone marrow.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Improved co-expression of multiple genes in vectors containing internal ribosome entry sites (IRESes) from human genes.Gene therapy · 2002Article
- Improved post-transcriptional processing of an MDR1 retrovirus elevates expression of multidrug resistance in primary human hematopoietic cells.Gene therapy · 2001Article
- Detection of recombinant P-glycoprotein in multidrug resistant cultured cells.Molecular biotechnology · 2000Article
- Drug selection of MDR1-transduced hematopoietic cells ex vivo increases transgene expression and chemoresistance in reconstituted bone marrow in mice.Gene therapy · 2000Article
- Bicistronic retroviral vectors for combining myeloprotection with cell-surface marking.Gene therapy · 1999Article
- A proliferation switch for genetically modified cells.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Development of a high-performance liquid chromatographic assay for G418 sulfate (Geneticin).Antimicrobial agents and chemotherapy · 1997Article
- Selection of transduced CD34+ progenitors and enzymatic correction of cells from Gaucher patients, with bicistronic vectors.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Genetic elements of plant viruses as tools for genetic engineering.Microbiological reviews · 1995Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bicistronic cassettes under control of a single promoter have recently been suggested as useful tools for coordinate expression of two different foreign proteins in mammalian cells. Using the long 5' untranslated region of encephalomyocarditis virus as translational enhancer of the second gene, a bicistronic unit composed of cDNA for human P-glycoprotein [the product of the multidrug resistance gene, MDR1 (also called PGY1)] as selectable marker and cDNA for human glucocerebrosidase (GC; EC 3.2.1.45) (a membrane-associated lysosomal hydrolase) was constructed. NIH 3T3 cells transfected with a Harvey murine sarcoma virus retroviral vector carrying this bicistronic cassette (pHaMCG) express active P-glycoprotein and GC and expression of both proteins augments coordinately with selection for increased colchicine resistance. Percoll gradient analysis of homogenates showed that GC was targeted to the lysosomal fraction. The ability to select for expression of GC with natural product drugs after introduction of the pHaMCG retroviral vector may be useful in gene therapy strategies for Gaucher disease.
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