ArticleMolecular and cellular biology1994
Coordinated trans activation of DNA synthesis- and precursor-producing enzymes by polyomavirus large T antigen through interaction with the retinoblastoma protein.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- EAPP, a novel E2F binding protein that modulates E2F-dependent transcription.Molecular biology of the cell · 2005Article
- Activation of CREB/ATF sites by polyomavirus large T antigen.Journal of virology · 2005Article
- Polyomavirus tumorantigens have a profound effect on gene expression in mouse fibroblasts.Oncogene · 2004Article
- Discrete signaling pathways participate in RB-dependent responses to chemotherapeutic agents.Oncogene · 2004Article
- Transactivation of murine cyclin A by polyomavirus large and small T antigens.Journal of virology · 2001Article
- J domain-independent regulation of the Rb family by polyomavirus large T antigen.Journal of virology · 2000Article
- Binding of polyomavirus small T antigen to protein phosphatase 2A is required for elimination of p27 and support of S-phase induction in concert with large T antigen.Journal of virology · 1999Article
- The retinoblastoma protein alters the phosphorylation state of polyomavirus large T antigen in murine cell extracts and inhibits polyomavirus origin DNA replication.Journal of virology · 1999Article
- Polyomavirus large T antigen binds the transcriptional coactivator protein p300.Journal of virology · 1999Article
- The activity of differentiation factors induces apoptosis in polyomavirus large T-expressing myoblasts.Molecular biology of the cell · 1998Article
- The DnaJ domain of polyomavirus large T antigen is required to regulate Rb family tumor suppressor function.Journal of virology · 1997Article
- Phosphorylation sites in polyomavirus large T antigen that regulate its function in viral, but not cellular, DNA synthesis.Journal of virology · 1997Article
- Glucocorticoid receptor-mediated cell cycle arrest is achieved through distinct cell-specific transcriptional regulatory mechanisms.Molecular and cellular biology · 1997Article
- Induction of cyclins E and A in response to mitogen removal: a basic alteration associated with the arrest of differentiation of C2 myoblasts transformed by simian virus 40 large T antigen.Journal of virology · 1997Article
- Activation of the human thymidine kinase (TK) promoter by simian virus 40 large T antigen requires both the T antigen pRb family-binding domain and TK promoter sequences resembling E2F-binding sites.Journal of virology · 1996Article
- Genetic analysis of polyomavirus large T nuclear localization: nuclear localization is required for productive association with pRb family members.Journal of virology · 1996Article
- Identification of leukaemic cells in bone marrow and blood samples by a new cytofluorometric assay.British journal of cancer · 1996Article
- E2F-1 blocks terminal differentiation and causes proliferation in transgenic megakaryocytes.Molecular and cellular biology · 1996Article
- A geminivirus induces expression of a host DNA synthesis protein in terminally differentiated plant cells.The Plant cell · 1995Article
- Protein domains connect cell cycle stimulation directly to initiation of DNA replication.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously constructed Swiss mouse 3T3 fibroblasts producing polyomavirus large T antigen after addition of dexamethasone were used to study the transcriptional activation by the viral protein of five genes coding for enzymes involved in DNA synthesis and precursor production, namely, dihydrofolate reductase, thymidine kinase, thymidylate synthase, DNA polymerase alpha, and proliferating-cell nuclear antigen. It was found that all these genes, whose expression is stimulated at the G1/S boundary of the cell cycle after growth stimulation by serum addition, are coordinately trans activated when T antigen is induced in cells previously growth arrested by serum withdrawal. Cell lines carrying the information for a mutant form of large T antigen, in which a glutamic acid residue in the binding site for the retinoblastoma protein was changed into aspartic acid, were constructed to test the involvement of an interaction of T antigen with the retinoblastoma protein in this reaction. It was found that the mutated T protein is incapable of stimulating transcription of any one of the genes. The promoter of three of the genes (dihydrofolate reductase, thymidine kinase, and DNA polymerase alpha) unequivocally carries binding sites for transcription factor E2F, suggesting that complexes forming with this growth- and cell cycle-regulating transcription factor are the targets for T antigen. Although there is so far no evidence that thymidylate synthase and proliferating cell nuclear antigen are regulated via E2F, our data indicate that the retinoblastoma protein still is involved in the control of these genes. mRNA for E2F itself increases in amount at the G1/S border in serum-stimulated cells but not during polyomavirus T antigen-induced transcriptional activation of DNA synthesis enzymes in arrested cells.
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