ArticleProceedings of the National Academy of Sciences of the United States of America1994
Cysteine-rich LIM domains of LIM-homeodomain and LIM-only proteins contain zinc but not iron.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 58 citations in OpenAlex.
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- Maturation strategy influences expression levels and cofactor occupancy in Fe-S proteins.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2023Article
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- Asp1 from Schizosaccharomyces pombe binds a [2Fe-2S](2+) cluster which inhibits inositol pyrophosphate 1-phosphatase activity.Biochemistry · 2015Article
- LMO2 at 25 years: a paradigm of chromosomal translocation proteins.Open biology · 2015Review
- Enforced expression of E47 has differential effects on Lmo2-induced T-cell leukemias.Leukemia research · 2015Article
- Conformational flexibility of the oncogenic protein LMO2 primes the formation of the multi-protein transcription complex.Scientific reports · 2014Article
- Single domain intracellular antibodies from diverse libraries: emphasizing dual functions of LMO2 protein interactions using a single VH domain.The Journal of biological chemistry · 2011Article
- GTPase-activating proteins for Cdc42.Eukaryotic cell · 2002Article
- Distinct iron-sulfur cluster assembly complexes exist in the cytosol and mitochondria of human cells.The EMBO journal · 2000Article
- The molecular basis for the role of zinc in developmental biology.Molecular and cellular biochemistry · 1998Review
- LIM domains of cysteine-rich protein 1 (CRP1) are essential for its zyxin-binding function.The Biochemical journal · 1998Article
- Expression of LIM protein genes Lmo1, Lmo2, and Lmo3 in adult mouse hippocampus and other forebrain regions: differential regulation by seizure activity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Transcriptional synergy between LIM-homeodomain proteins and basic helix-loop-helix proteins: the LIM2 domain determines specificity.Molecular and cellular biology · 1997Article
- Molecular dissection of a LIM domain.Molecular biology of the cell · 1997Article
- The LIM domain-containing Dbm1 GTPase-activating protein is required for normal cellular morphogenesis in Saccharomyces cerevisiae.Molecular and cellular biology · 1996Article
- Association of erythroid transcription factors: complexes involving the LIM protein RBTN2 and the zinc-finger protein GATA1.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Specific in vivo association between the bHLH and LIM proteins implicated in human T cell leukemia.The EMBO journal · 1994Article
- The LIM protein RBTN2 and the basic helix-loop-helix protein TAL1 are present in a complex in erythroid cells.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The structure of LIM domains has major implications for transcription because proteins such as Is1-1 contain two LIM domains associated with a homeodomain, and RBTN1/Ttg-1 and RBTN2/Ttg-2 contain two LIM domains but no homeodomain. Conserved cysteine and histidine residues in the LIM domains suggest a metal-binding role. RBTN and Is1-1 LIM proteins have been made in Escherichia coli and insect cell expression systems and their metal content has been determined using atomic absorption spectroscopy and electron paramagnetic resonance spectroscopy. LIM proteins expressed in soluble form contain zinc atoms, whereas bacterial inclusion bodies invariably also have Fe-S clusters. The latter are identified as linear [Fe3S4]+ clusters and appear to result from incorrect metal coordination by E. coli. These studies show that RBTN1, RBTN2, and Is1-1 are metalloproteins that contain zinc but not iron and, therefore, that the LIM domain represents a zinc-binding domain.
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