ArticleThe Journal of cell biology1993
Non-chemotactic Dictyostelium discoideum mutants with altered cGMP signal transduction.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The trial behind it
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Who cites it
28 citing papers in PubMed, 80 citations in OpenAlex.
- A Mutant of Dictyostelium discoideum, KI-Cell, as a Model of Collective Cell Migration Independent of Chemotaxis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Forty-five years of cGMP research inMolecular biology of the cell · 2021Review
- Polar pattern formation induced by contact following locomotion in a multicellular system.eLife · 2020Article
- Self-Generated Chemoattractant Gradients: Attractant Depletion Extends the Range and Robustness of Chemotaxis.PLoS biology · 2016Article
- A RabGAP regulates life-cycle duration via trimeric G-protein cascades in Dictyostelium discoideum.PloS one · 2013Article
- Biological soliton in multicellular movement.Scientific reports · 2013Article
- Arachidonic acid enhances caffeine-induced cell death via caspase-independent cell death.Scientific reports · 2012Article
- Open access microfluidic device for the study of cell migration during chemotaxis.Integrative biology : quantitative biosciences from nano to macro · 2010Article
- Differentiation-inducing factor-1 and -2 function also as modulators for Dictyostelium chemotaxis.PloS one · 2009Article
- Switching direction in electric-signal-induced cell migration by cyclic guanosine monophosphate and phosphatidylinositol signaling.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Phosducin-like proteins in Dictyostelium discoideum: implications for the phosducin family of proteins.The EMBO journal · 2003Article
- Identification and characterization of two unusual cGMP-stimulated phoshodiesterases in dictyostelium.Molecular biology of the cell · 2002Article
- Identification of a novel type of cGMP phosphodiesterase that is defective in the chemotactic stmF mutants.Molecular biology of the cell · 2002Article
- A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium.The EMBO journal · 2002Article
- Guanylate cyclase in Dictyostelium discoideum with the topology of mammalian adenylate cyclase.The Biochemical journal · 2001Article
- Tortoise, a novel mitochondrial protein, is required for directional responses of Dictyostelium in chemotactic gradients.The Journal of cell biology · 2001Article
- Ca(2+) signalling is not required for chemotaxis in Dictyostelium.The EMBO journal · 2000Article
- Selection of gbeta subunits with point mutations that fail to activate specific signaling pathways in vivo: dissecting cellular responses mediated by a heterotrimeric G protein in Dictyostelium discoideum.Molecular biology of the cell · 1998Article
- A novel cytosolic regulator, Pianissimo, is required for chemoattractant receptor and G protein-mediated activation of the 12 transmembrane domain adenylyl cyclase in Dictyostelium.Genes & development · 1997Article
- Chemotaxis to cAMP and slug migration in Dictyostelium both depend on migA, a BTB protein.Molecular biology of the cell · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Folic acid and cAMP are chemoattractants in Dictyostelium discoideum, which bind to different surface receptors. The signal is transduced from the receptors via different G proteins into a common pathway which includes guanylyl cyclase and acto-myosin. To investigate this common pathway, ten mutants which do not react chemotactically to both cAMP and folic acid were isolated with a simple new chemotactic assay. Genetic analysis shows that one of these mutants (KI-10) was dominant; the other nine mutants were recessive, and comprise nine complementation groups. In wild-type cells, the chemoattractants activate adenylyl cyclase, phospholipase C, and guanylyl cyclase in a transient manner. In mutant cells the formation of cAMP and IP3 were generally normal, whereas the cGMP response was altered in most of the ten mutants. Particularly, mutant KI-8 has strongly reduced basal guanylyl cyclase activity; the enzyme is present in mutant KI-10, but can not be activated by cAMP or folic acid. The cGMP response of five other mutants is altered in either magnitude, dose dependency, or kinetics. These observations suggest that the second messenger cGMP plays a key role in chemotaxis in Dictyostelium.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.