ArticleThe Journal of cell biology1993
The synaptic vesicle proteins SV2, synaptotagmin and synaptophysin are sorted to separate cellular compartments in CHO fibroblasts.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 72 citations in OpenAlex.
- Article
- SGIP1α functions as a selective endocytic adaptor for the internalization of synaptotagmin 1 at synapses.Molecular brain · 2019Article
- Safeguards of Neurotransmission: Endocytic Adaptors as Regulators of Synaptic Vesicle Composition and Function.Frontiers in cellular neuroscience · 2017Review
- Alternative splicing of neuroligin and its protein distribution in the outer plexiform layer of the chicken retina.The Journal of comparative neurology · 2010Article
- Cerebellar neurons possess a vesicular compartment structurally and functionally similar to Glut4-storage vesicles from peripheral insulin-sensitive tissues.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Synaptophysin I selectively specifies the exocytic pathway of synaptobrevin 2/VAMP2.The Biochemical journal · 2007Article
- ZNRF proteins constitute a family of presynaptic E3 ubiquitin ligases.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- The AP2 binding site of synaptotagmin 1 is not an internalization signal but a regulator of endocytosis.The Journal of cell biology · 2001Article
- A conserved mechanism of synaptogyrin localization.Molecular biology of the cell · 2001Article
- Synaptotagmin VII regulates Ca(2+)-dependent exocytosis of lysosomes in fibroblasts.The Journal of cell biology · 2000Article
- Advances in animal cell recombinant protein production: GS-NS0 expression system.Cytotechnology · 2000Article
- Vesicular neurotransmitter transporters. Potential sites for the regulation of synaptic function.Molecular neurobiology · 1997Review
- Patterns of synaptic activity in neural networks recorded by light emission from synaptolucins.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Targeting of an intestinal apical endosomal protein to endosomes in nonpolarized cells.The Journal of cell biology · 1997Article
- Targeting of P-selectin to two regulated secretory organelles in PC12 cells.The Journal of cell biology · 1996Article
- Endocytosis of VAMP is facilitated by a synaptic vesicle targeting signal.The Journal of cell biology · 1996Article
- Structure of synaptogyrin (p29) defines novel synaptic vesicle protein.The Journal of cell biology · 1995Article
- Biogenesis of synaptic vesicles in vitro.The Journal of cell biology · 1995Article
- KIF2 is a new microtubule-based anterograde motor that transports membranous organelles distinct from those carried by kinesin heavy chain or KIF3A/B.The Journal of cell biology · 1995Article
- Sorting of synaptophysin into special vesicles in nonneuroendocrine epithelial cells.The Journal of cell biology · 1994Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We expressed the synaptic vesicle proteins SV2, synaptotagmin, and synaptophysin in CHO fibroblasts to investigate the targeting information contained by each protein. All three proteins entered different cellular compartments. Synaptotagmin was found on the plasma membrane. Both SV2 and synaptophysin were sorted to small intracellular vesicles, but synaptophysin colocalized with early endosomal markers, while SV2 did not. SV2-containing vesicles did not have the same sedimentation characteristics as authentic synaptic vesicles, even though transfected SV2 was sorted from endosomal markers. We also created cell lines expressing both SV2 and synaptotagmin, both synaptotagmin and synaptophysin, and lines expressing all three synaptic vesicle proteins. In all cases, the proteins maintained their distinct compartmentalizations, were not found in the same organelle, and did not created synaptic vesicle-like structures. These results have important implications for models of synaptic vesicle biogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.