ArticleThe EMBO journal1993
The cysteine-rich LIM domains inhibit DNA binding by the associated homeodomain in Isl-1.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 100 citations in OpenAlex.
- Overexpression of LIM Homeodomain GeneBiology · 2025Article
- Spotlight on Isl1: A Key Player in Cardiovascular Development and Diseases.Frontiers in cell and developmental biology · 2021Review
- Protein Inhibitor of Activated STAT Y (PIASy) Regulates Insulin Secretion by Interacting with LIM Homeodomain Transcription Factor Isl1.Scientific reports · 2016Article
- Functional Diversification of Motor Neuron-specific Isl1 Enhancers during Evolution.PLoS genetics · 2015Article
- LIM homeodomain transcription factor Isl1 directs normal pyloric development by targeting Gata3.BMC biology · 2014Article
- A structural basis for the regulation of the LIM-homeodomain protein islet 1 (Isl1) by intra- and intermolecular interactions.The Journal of biological chemistry · 2013Article
- Liver-specific Ldb1 deletion results in enhanced liver cancer development.Journal of hepatology · 2010Article
- Article
- Pxl1p, a paxillin-like protein in Saccharomyces cerevisiae, may coordinate Cdc42p and Rho1p functions during polarized growth.Molecular biology of the cell · 2004Article
- LIM-domain-binding protein 1: a multifunctional cofactor that interacts with diverse proteins.EMBO reports · 2003Review
- Transcriptional activation by the PHD finger is inhibited through an adjacent leucine zipper that binds 14-3-3 proteins.Nucleic acids research · 2000Article
- The level of DLDB/CHIP controls the activity of the LIM homeodomain protein apterous: evidence for a functional tetramer complex in vivo.The EMBO journal · 2000Article
- Cofilin phosphorylation and actin cytoskeletal dynamics regulated by rho- and Cdc42-activated LIM-kinase 2.The Journal of cell biology · 1999Article
- The N-terminal LIM domain negatively regulates the kinase activity of LIM-kinase 1.The Biochemical journal · 1999Article
- Beadex encodes an LMO protein that regulates Apterous LIM-homeodomain activity in Drosophila wing development: a model for LMO oncogene function.Genes & development · 1998Article
- The Drosophila LIM-only gene, dLMO, is mutated in Beadex alleles and might represent an evolutionarily conserved function in appendage development.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- The LIM-domain binding protein Ldb1 and its partner LMO2 act as negative regulators of erythroid differentiation.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Chip, a widely expressed chromosomal protein required for segmentation and activity of a remote wing margin enhancer in Drosophila.Genes & development · 1997Article
- Functional analysis of the nuclear LIM domain interactor NLI.Molecular and cellular biology · 1997Article
- Expression of LIM protein genes Lmo1, Lmo2, and Lmo3 in adult mouse hippocampus and other forebrain regions: differential regulation by seizure activity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently, a new class of homeobox genes has been identified, called LIM-homeobox genes. These genes encode proteins which have two tandemly repeated cysteine motifs, referred to as LIM domains, in addition to a homeodomain. In addition, proteins with only LIM domains have been described but the function of the LIM domain is unknown. We have analysed the function of LIM domains using Isl-1 as a representative LIM-homeodomain protein. Employing protein prepared in bacterial cells, we show that the presence of the LIM domain in Isl-1 inhibits binding of the homeodomain to its DNA target. This in vitro inhibition can be released either by denaturation/renaturation of the protein or by truncation of the LIM domains. A similar inhibition is observed in vivo using reporter constructs. In addition we show that LIM domains in a chimeric protein can inhibit binding of the Ubx homeodomain to its target. The ability of LIM domains to inhibit DNA binding by the homeodomain provides a possible basis for negative regulation of LIM-homeodomain proteins in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.