ArticleMolecular & general genetics : MGG1995
Clustered alpha-amanitin resistance mutations in mouse.
Article in Molecular & general genetics : MGG, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 31 citations in OpenAlex.
- Cryo-EM structure of a mammalian RNA polymerase II elongation complex inhibited by α-amanitin.The Journal of biological chemistry · 2018Article
- Amino Acid Substitutions in the Caenorhabditis elegans RNA Polymerase II Large Subunit AMA-1/RPB-1 that Result in α-Amanitin Resistance and/or Reduced Function.G3 (Bethesda, Md.) · 2011Article
- Inhibiting eukaryotic transcription: Which compound to choose? How to evaluate its activity?Transcription · 2011Article
- Phylogeny of parasitic parabasalia and free-living relatives inferred from conventional markers vs. Rpb1, a single-copy gene.PloS one · 2011Article
- Comparative whole genome sequence analysis of wild-type and cidofovir-resistant monkeypoxvirus.Virology journal · 2010Article
- Structural perspective on mutations affecting the function of multisubunit RNA polymerases.Microbiology and molecular biology reviews : MMBR · 2006Review
- Transcription-coupled and DNA damage-dependent ubiquitination of RNA polymerase II in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Structural basis of transcription: alpha-amanitin-RNA polymerase II cocrystal at 2.8 A resolution.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Comparison of the RNA polymerase III transcription machinery in Schizosaccharomyces pombe, Saccharomyces cerevisiae and human.Nucleic acids research · 2001Review
- A genetic look at the active site of RNA polymerase III.EMBO reports · 2001Article
- Trypanosome spliced leader RNA genes contain the first identified RNA polymerase II gene promoter in these organisms.Nucleic acids research · 2001Article
- Stimulation of transcription by mutations affecting conserved regions of RNA polymerase II.Journal of bacteriology · 1998Article
- The largest subunit of human RNA polymerase III is closely related to the largest subunit of yeast and trypanosome RNA polymerase III.Genome research · 1997Article
- In vivo degradation of RNA polymerase II largest subunit triggered by alpha-amanitin.Nucleic acids research · 1996Article
- Mutations in the alpha-amanitin conserved domain of the largest subunit of yeast RNA polymerase III affect pausing, RNA cleavage and transcriptional transitions.The EMBO journal · 1996Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the identification of three new alpha-amanitin resistance mutations in the gene encoding the largest subunit of mouse RNA polymerase II (RPII215). These mutations are clustered in a region of the largest subunit that is important for transcription elongation. This same domain has been identified as the site of alpha-amanitin resistance mutations in both Drosophila and Caenarhabditis elegans. The sequences encompassing this cluster of mutations are highly conserved among RNA polymerase II genes from a number of species, including those that are naturally more resistant to alpha-amanitin suggesting that this region of the largest subunit is critical for a conserved catalytic function. The mutations reported here change leucine 745 to phenylalanine, arginine 749 to proline, or isoleucine 779 to phenylalanine. Together with the previously reported asparagine 792 to aspartate substitution these mutations define a potential alpha-amanitin binding pocket in a region of the mouse subunit that could be involved in translocation of polymerase during elongation.
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