ArticleMolecular & general genetics : MGG1995
Reactivation of the ATCase domain of the URA2 gene complex: a positive selection method for Ty insertions and chromosomal rearrangements in Saccharomyces cerevisiae.
Article in Molecular & general genetics : MGG, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed, 21 citations in OpenAlex.
- Ploidy influences cellular responses to gross chromosomal rearrangements in Saccharomyces cerevisiae.BMC genomics · 2011Article
- Influence of genetic background on the occurrence of chromosomal rearrangements in Saccharomyces cerevisiae.BMC genomics · 2009Article
- Segmental duplications arise from Pol32-dependent repair of broken forks through two alternative replication-based mechanisms.PLoS genetics · 2008Article
- Duplication processes in Saccharomyces cerevisiae haploid strains.Nucleic acids research · 2005Article
- Recovery of a function involving gene duplication by retroposition in Saccharomyces cerevisiae.Genome research · 2004Article
- Eucaryotic genome evolution through the spontaneous duplication of large chromosomal segments.The EMBO journal · 2004Article
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4 authors at 1 institution in 1 country.
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Abstract
Genetic rearrangements such as deletions or duplications of DNA sequences are rarely detected in the yeast Saccharomyces cerevisiae. We have developed a screening system using the URA2 gene coding for the bi-functional CPSase-ATCase (carbamyl phosphate synthetase - aspartate transcarbamylase) to select positively for these kinds of events. Nonsense mutations in the CPSase region cause a complete loss of the ATCase activity because of their strong polar effect. Thirty-seven ATCase+ revertants were isolated from a strain containing three nonsense mutations in the proximal CPSase region. Genetic and structural analysis of the URA2 locus in these strains allowed us to characterize two major classes of revertants. In the first, an entire copy of a Ty transposon was found to be inserted in the CPSase coding domain. This event, which represents a new form of Ty-mediated gene activation was further analysed by mapping the Ty integration site in 26 strains. In a second class of revertants, we observed chromosomal rearrangements and, in particular, duplication of the ATCase region and its integration in a new chromosomal environment in which this sequence becomes active.
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