ArticleThe Journal of cell biology1995
Molecular cloning, expression, and mapping of the high affinity actin-capping domain of chicken cardiac tensin.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 61 citations in OpenAlex.
- Tensin3 interaction with talin drives the formation of fibronectin-associated fibrillar adhesions.The Journal of cell biology · 2022Article
- Tensins - emerging insights into their domain functions, biological roles and disease relevance.Journal of cell science · 2021Review
- Tensin regulates pharyngeal pumping in Caenorhabditis elegans.Biochemical and biophysical research communications · 2020Article
- Tensin1 expression and function in chronic obstructive pulmonary disease.Scientific reports · 2019Article
- Integrin-bound talin head inhibits actin filament barbed-end elongation.The Journal of biological chemistry · 2018Article
- Forecast of actin-binding proteins as the oncotarget in osteosarcoma - a review of mechanism, diagnosis and therapy.OncoTargets and therapy · 2018Review
- CpG dinucleotide-specific hypermethylation of the TNS3 gene promoter in human renal cell carcinoma.Epigenetics · 2013Article
- Vinculin is a dually regulated actin filament barbed end-capping and side-binding protein.The Journal of biological chemistry · 2010Article
- Tensin 2 modulates cell contractility in 3D collagen gels through the RhoGAP DLC1.Journal of cellular biochemistry · 2010Article
- Small heat shock proteins in smooth muscle.Pharmacology & therapeutics · 2008Review
- Structure of the PTB domain of tensin1 and a model for its recruitment to fibrillar adhesions.Protein science : a publication of the Protein Society · 2007Article
- Staphylococcus aureus fibronectin binding protein-A induces motile attachment sites and complex actin remodeling in living endothelial cells.Molecular biology of the cell · 2006Article
- Tensin is potentially involved in extracellular matrix production in mesangial cells.Histochemistry and cell biology · 2004Article
- Tensin1 and a previously undocumented family member, tensin2, positively regulate cell migration.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- A role of tensin in skeletal-muscle regeneration.The Biochemical journal · 2001Article
- Molecular characterization of human tensin.The Biochemical journal · 2000Article
- Inhibition of integrin-linked kinase (ILK) suppresses activation of protein kinase B/Akt and induces cell cycle arrest and apoptosis of PTEN-mutant prostate cancer cells.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Integrin dynamics and matrix assembly: tensin-dependent translocation of alpha(5)beta(1) integrins promotes early fibronectin fibrillogenesis.The Journal of cell biology · 2000Article
- A 29 kDa intracellular chloride channel p64H1 is associated with large dense-core vesicles in rat hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Regulation of G1 progression by the PTEN tumor suppressor protein is linked to inhibition of the phosphatidylinositol 3-kinase/Akt pathway.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Tensin, an actin filament capping protein first purified from chicken gizzard, is localized to various types of adherens junctions in muscle and nonmuscle cells. In this paper, we describe the isolation and sequencing of tensin cDNA from a chicken cardiac library. The 6.3-kb chicken cardiac tensin cDNA encodes an open reading frame of 1,792 amino acids. Mammalian cells transfected with the chicken tensin cDNA expressed a polypeptide of approximately 200 kD recognizable by antibodies to chicken gizzard tensin. The expressed protein was incorporated into focal adhesions and other actin-containing structures in the transfected cells. To map the domain associated with tensin's high affinity, barbed-end F-actin-capping activity, bacterially expressed recombinant fusion proteins containing various segments of tensin were prepared and assayed for activity. The results of these experiments show that the high affinity capping domain (kD = 1.3 nM) lies within amino acid residues R1037-V1169. Additional studies on a shorter construct, S1061-H1145, showed that these 85 residues were sufficient for producing complete inhibition of actin polymerization and depolymerization. While this active domain is located within that of the "insertin" sequence (Weigt, C., A. Gaertner, A. Wegner, H. Korte, and H. E. Meyer. 1992. J. Mol. Biol. 227:593-595), our data showing complete inhibition of polymerization and shift in critical concentration are consistent with a simple barbed-end capping mechanism rather than the "insertin model." Our results also differ from those of a recent report (Lo, S. H., P. A. Janmey, J. H. Hartwig, and L. B. Chen. 1994. J. Cell Biol. 125:1067-1075), which concluded that their recombinant tensin has an "insertin-like" inhibitory effect on barbed-end actin polymerization, and that this activity is attributed to residues T936-R1037 (residues 888-989 in their numbering system). In our study, a fusion construct (N790-K1060) encompassing T936-R1037 had no significant effect on actin polymerization and depolymerization, even at high concentrations.
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