ArticleThe Journal of cell biology1995
SNAP-25 is expressed in islets of Langerhans and is involved in insulin release.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 244 citations in OpenAlex.
- Gβγ-SNAP25 exocytotic brake removal enhances insulin action, promotes adipocyte browning, and protects against diet-induced obesity.The Journal of clinical investigation · 2023Article
- SNAP25 mutation disrupts metabolic homeostasis, steroid hormone production and central neurobehavior.Biochimica et biophysica acta. Molecular basis of disease · 2022Article
- Vertical sleeve gastrectomy triggers fast β-cell recovery upon overt diabetes.Molecular metabolism · 2021Article
- Syntaxin 4 Mediates NF-κB Signaling and Chemokine Ligand Expression via Specific Interaction With IκBβ.Diabetes · 2021Article
- SNAP23 depletion enables more SNAP25/calcium channel excitosome formation to increase insulin exocytosis in type 2 diabetes.JCI insight · 2020Article
- ARHGAP21 Acts as an Inhibitor of the Glucose-Stimulated Insulin Secretion Process.Frontiers in endocrinology · 2020Article
- Syntaxin 4 Expression in Pancreatic β-Cells Promotes Islet Function and Protects Functional β-Cell Mass.Diabetes · 2018Article
- Exocytosis proteins as novel targets for diabetes prevention and/or remediation?American journal of physiology. Regulatory, integrative and comparative physiology · 2017Review
- Neuron-enriched RNA-binding Proteins Regulate Pancreatic Beta Cell Function and Survival.The Journal of biological chemistry · 2017Article
- Opposing roles for SNAP23 in secretion in exocrine and endocrine pancreatic cells.The Journal of cell biology · 2016Article
- Synaptosomal Protein of 25 kDa (Snap25) Polymorphisms Associated with Glycemic Parameters in Type 2 Diabetes Patients.Journal of diabetes research · 2016Article
- BAG3 regulates formation of the SNARE complex and insulin secretion.Cell death & disease · 2015Article
- Effect of botulinum toxin A on urothelial-release of ATP and expression of SNARE targets within the urothelium.Neurourology and urodynamics · 2015Article
- Nova1 is a master regulator of alternative splicing in pancreatic beta cells.Nucleic acids research · 2014Article
- Distinct actions of Rab3 and Rab27 GTPases on late stages of exocytosis of insulin.Traffic (Copenhagen, Denmark) · 2014Article
- Article
- Signaling mechanisms of glucose-induced F-actin remodeling in pancreatic islet β cells.Experimental & molecular medicine · 2013Review
- Pharmacogenetics of antipsychotic-induced weight gain: review and clinical implications.Molecular psychiatry · 2012Review
- Novel mechanistic link between focal adhesion remodeling and glucose-stimulated insulin secretion.The Journal of biological chemistry · 2012Article
- Gelsolin associates with the N terminus of syntaxin 4 to regulate insulin granule exocytosis.Molecular endocrinology (Baltimore, Md.) · 2012Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
SNAP-25 is known as a neuron specific molecule involved in the fusion of small synaptic vesicles with the presynaptic plasma membrane. By immunolocalization and Western blot analysis, it is now shown that SNAP-25 is also expressed in pancreatic endocrine cells. Botulinum neurotoxins (BoNT) A and E were used to study the role of SNAP-25 in insulin secretion. These neurotoxins inhibit transmitter release by cleaving SNAP-25 in neurons. Cells from a pancreatic B cell line (HIT) and primary rat islet cells were permeabilized with streptolysin-O to allow toxin entry. SNAP-25 was cleaved by BoNT/A and BoNT/E, resulting in a molecular mass shift of approximately 1 and 3 kD, respectively. Cleavage was accompanied by an inhibition of Ca(++)-stimulated insulin release in both cell types. In HIT cells, a concentration of 30-40 nM BoNT/E gave maximal inhibition of stimulated insulin secretion of approximately 60%, coinciding with essentially complete cleavage of SNAP-25. Half maximal effects in terms of cleavage and inhibition of insulin release were obtained at a concentration of 5-10 nM. The A type toxin showed maximal and half-maximal effects at concentrations of 4 and 2 nM, respectively. In conclusion, the results suggest a role for SNAP-25 in fusion of dense core secretory granules with the plasma membrane in an endocrine cell type- the pancreatic B cell.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.