ArticleProceedings of the National Academy of Sciences of the United States of America1995
Cloning, expression, and characterization of cDNAs encoding Arabidopsis thaliana squalene synthase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 99 citations in OpenAlex.
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- Analysis of Biosynthetic Gene Clusters, Secretory, and Antimicrobial Peptides Reveals Environmental Suitability ofFrontiers in microbiology · 2021Article
- Origin and Evolution of Polycyclic Triterpene Synthesis.Molecular biology and evolution · 2020Article
- Bioactive compounds induced in Physalis angulata L. by methyl-jasmonate: an investigation of compound accumulation patterns and biosynthesis-related candidate genes.Plant molecular biology · 2020Article
- Molecular Cloning and Functional Identification of a Squalene Synthase Encoding Gene from Alfalfa (International journal of molecular sciences · 2019Article
- Squalene synthase cloning and functional identification in wintersweet plant (Chimonanthus zhejiangensis).Botanical studies · 2018Article
- Article
- Production of squalene by microbes: an update.World journal of microbiology & biotechnology · 2016Review
- Quantitative Gene Expression of ERG9 in Model Saccharomyces cerevisiae: Chamomile Extract For Human Cancer Treatment.Journal of clinical and diagnostic research : JCDR · 2016Article
- Directed optimization of a newly identified squalene synthase from Mortierella alpine based on sequence truncation and site-directed mutagenesis.Journal of industrial microbiology & biotechnology · 2015Article
- Article
- Chrysanthemyl diphosphate synthase operates in planta as a bifunctional enzyme with chrysanthemol synthase activity.The Journal of biological chemistry · 2014Article
- Molecular cloning and differential expression analysis of a squalene synthase gene from Dioscorea zingiberensis, an important pharmaceutical plant.Molecular biology reports · 2014Article
- Construction and analysis of EST libraries of the trans-polyisoprene producing plant, Eucommia ulmoides Oliver.Planta · 2012Article
- Cloning and characterization of squalene synthase gene from Fusarium fujikuroi (Saw.) Wr.Journal of industrial microbiology & biotechnology · 2010Article
- Cloning and characterization of a squalene synthase gene from a petroleum plant, Euphorbia tirucalli L.Planta · 2009Article
- Cloning, solubilization, and characterization of squalene synthase from Thermosynechococcus elongatus BP-1.Journal of bacteriology · 2008Article
- Arabidopsis thaliana contains a single gene encoding squalene synthase.Plant molecular biology · 2008Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have isolated and characterized two overlapping cDNA clones for Arabidopsis thaliana squalene synthase. Their nucleotide sequences contained an open reading frame for a 410-amino acid polypeptide (calculated molecular mass, 47 kDa). The deduced amino acid sequence of the Arabidopsis polypeptide was significantly homologous (42-44% identical) to the sequences of known squalene synthases of several species, from yeast to man, but it was much less homologous to that of tomato phytoene synthase. To express the Arabidopsis enzyme in Escherichia coli, the entire coding region was subcloned into an expression vector. A cell-free extract of E. coli transformed with the recombinant plasmid, in the presence of NADPH and Mg2+, efficiently converted [14C]farnesyl diphosphate into squalene. On the other hand, in the absence of NADPH and the presence of Mn2+, the cell-free extract formed dehydrosqualene as a secondary product. Another E. coli extract expressing mouse squalene synthase showed the same activity as the Arabidopsis enzyme. Therefore, both the structure and reaction mechanism of squalene synthases are markedly conserved in taxonomically remote eukaryotes.
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