ArticleProceedings of the National Academy of Sciences of the United States of America1995
A method for probing the topography and interactions of proteins: footprinting of myoglobin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed.
- A Hybrid Structural Method for Investigating Low Molecular Weight Oligomeric Structures of Amyloid Beta.Chembiochem : a European journal of chemical biology · 2022Article
- Oxidative functionalization of aliphatic and aromatic amino acid derivatives with HRSC advances · 2018Article
- Laser-Initiated Radical Trifluoromethylation of Peptides and Proteins: Application to Mass-Spectrometry-Based Protein Footprinting.Angewandte Chemie (International ed. in English) · 2017Article
- Protein footprinting in a complex milieu: identifying the interaction surfaces of the chemotaxis adaptor protein CheW.Journal of molecular biology · 2011Article
- Oxidative protein labeling in mass-spectrometry-based proteomics.Analytical and bioanalytical chemistry · 2010Review
- Isotope-coded affinity tags with tunable reactivities for protein footprinting.Angewandte Chemie (International ed. in English) · 2008Article
- Conformational properties of native sperm whale apomyoglobin in solution.Protein science : a publication of the Protein Society · 1999Article
- Role of the S3-S4 linker in Shaker potassium channel activation.The Journal of general physiology · 1997Article
- Proteolytic mapping of heat shock transcription factor domains.Protein science : a publication of the Protein Society · 1996Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We describe a procedure for mapping residues on the surface of a protein molecule to its sequence, using a scheme that is analogous to nucleic acid footprinting. The protein is end labeled radioactively and subjected to limited proteolysis, and the products are analyzed by denaturing polyacrylamide gel electrophoresis and autoradiography. The method is tested with the heme protein myoglobin and applied to mapping the (unknown) surface of the molecule lacking the heme group: apomyoglobin. Sites of protein-protein interaction can be identified, as illustrated by footprinting the association between myoglobin and an anti-myoglobin monoclonal antibody.
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Registered trials
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