ArticleMolecular and cellular biology1995
Fine mapping of 28S rRNA sites specifically cleaved in cells undergoing apoptosis.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
46 citing papers in PubMed, 117 citations in OpenAlex.
- Ribosomal RNA Degradation (RNA Disruption) in Tumour Cells: Mechanistic Insights and Potential Clinical Utility.Cancers · 2025Review
- Exploring the protective role of caffeine against Taraxacum-Induced ribotoxic stress mediated through autophagy and mitochondrial depolarization.Scientific reports · 2025Article
- Diversity of ribosomes at the level of rRNA variation associated with human health and disease.Cell genomics · 2024Article
- Chemical Annealing Restructures RNA for Nanopore Detection.Journal of the American Chemical Society · 2024Article
- RNA disruption is a widespread phenomenon associated with stress-induced cell death in tumour cells.Scientific reports · 2023Article
- Cytoplasmic mRNA decay represses RNA polymerase II transcription during early apoptosis.eLife · 2021Article
- Cell Death-Associated Ribosomal RNA Cleavage in Postmortem Tissues and Its Forensic Applications.Molecules and cells · 2017Article
- Global decay of mRNA is a hallmark of apoptosis in aging Xenopus eggs.RNA biology · 2017Article
- Prime, Shock, and Kill: Priming CD4 T Cells from HIV Patients with a BCL-2 Antagonist before HIV Reactivation Reduces HIV Reservoir Size.Journal of virology · 2016Article
- RNA disruption is associated with response to multiple classes of chemotherapy drugs in tumor cell lines.BMC cancer · 2016Article
- Cell Death Inducing Microbial Protein Phosphatase Inhibitors--Mechanisms of Action.Marine drugs · 2015Review
- Effects of post-mortem and physical degradation on RNA integrity and quality.Biomolecular detection and quantification · 2015Article
- The properties of microparticles from RAW 264.7 macrophage cells undergoing in vitro activation or apoptosis.Innate immunity · 2014Article
- Ribonuclease L and metal-ion-independent endoribonuclease cleavage sites in host and viral RNAs.Nucleic acids research · 2014Article
- One core, two shells: bacterial and eukaryotic ribosomes.Nature structural & molecular biology · 2012Review
- Targets and intracellular signaling mechanisms for deoxynivalenol-induced ribosomal RNA cleavage.Toxicological sciences : an official journal of the Society of Toxicology · 2012Article
- Aberrant expression of miR-638 contributes to benzo(a)pyrene-induced human cell transformation.Toxicological sciences : an official journal of the Society of Toxicology · 2012Article
- Cleavage of rRNA ensures translational cessation in sperm at fertilization.Molecular human reproduction · 2011Article
- The post-apoptotic fate of RNAs identified through high-throughput sequencing of human hair.PloS one · 2011Article
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Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bona fide apoptosis in rat and human leukemia cells, rat thymocytes, and bovine endothelial cells was accompanied by limited and specific cleavage of polysome-associated and monosome-associated 28S rRNA, with 18S rRNA being spared. Specific 28S rRNA cleavage was observed in all instances of apoptotic death accompanied by internucleosomal DNA fragmentation, with cleavage of 28S rRNA and of DNA being linked temporally. This indicates that 28S rRNA fragmentation may be as general a feature of apoptosis as internucleosomal DNA fragmentation and that concerted specific cleavage of intra- and extranuclear polynucleotides occurs in apoptosis. Apoptosis-associated cleavage sites were mapped to the 28S rRNA divergent domains D2, D6 (endothelial cells), and D8. The D2 cuts occurred in hairpin loop junctions considered to be buried in the intact ribosome, suggesting that this rRNA region becomes a target for RNase attack in apoptotic cells. D8 was cleaved in two exposed UU(U) sequences in bulge loops. Treatment with agents causing necrotic cell death or aging of cell lysates failed to produce any detectable limited D2 cleavage but did produce a more generalized cleavage in the D8 region. Of potential functional interest was the finding that the primary cuts in D2 exactly flanked a 0.3-kb hypervariable subdomain (D2c), allowing excision of the latter. The implication of hypervariable rRNA domains in apoptosis represents the first association of any functional process with these enigmatic parts of the ribosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.