ArticleMolecular and cellular biology1995
Binding of a cell-type-specific RNA splicing factor to its target regulatory sequence.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- The SR-protein Npl3 is an essential component of the meiotic splicing regulatory network in Saccharomyces cerevisiae.Nucleic acids research · 2021Article
- Mechanism and Control of Meiotic DNA Double-Strand Break Formation inFrontiers in cell and developmental biology · 2021Review
- An essential role for trimethylguanosine RNA caps in Saccharomyces cerevisiae meiosis and their requirement for splicing of SAE3 and PCH2 meiotic pre-mRNAs.Nucleic acids research · 2011Article
- Determinants of Nam8-dependent splicing of meiotic pre-mRNAs.Nucleic acids research · 2011Article
- Integration of a splicing regulatory network within the meiotic gene expression program of Saccharomyces cerevisiae.Genes & development · 2010Article
- Spo11 and the Formation of DNA Double-Strand Breaks in Meiosis.Genome dynamics and stability · 2008Article
- Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae.Chromosoma · 2007Article
- YRA1 autoregulation requires nuclear export and cytoplasmic Edc3p-mediated degradation of its pre-mRNA.Molecular cell · 2007Article
- A subset of Mer1p-dependent introns requires Bud13p for splicing activation and nuclear retention.RNA (New York, N.Y.) · 2006Article
- Article
- Autoregulation of the mRNA export factor Yra1p requires inefficient splicing of its pre-mRNA.RNA (New York, N.Y.) · 2006Article
- The Saccharomyces cerevisiae gene CDC40/PRP17 controls cell cycle progression through splicing of the ANC1 gene.Nucleic acids research · 2004Article
- Support for a meiotic recombination initiation complex: interactions among Rec102p, Rec104p, and Spo11p.Molecular and cellular biology · 2003Article
- Expression of the essential mRNA export factor Yra1p is autoregulated by a splicing-dependent mechanism.RNA (New York, N.Y.) · 2002Article
- RNA-protein interactions that regulate pre-mRNA splicing.Gene expression · 2002Review
- Article
- Test of intron predictions reveals novel splice sites, alternatively spliced mRNAs and new introns in meiotically regulated genes of yeast.Nucleic acids research · 2000Article
- Splicing of the meiosis-specific HOP2 transcript utilizes a unique 5' splice site.Molecular and cellular biology · 1999Article
- Premature translation of oskar in oocytes lacking the RNA-binding protein bicaudal-C.Molecular and cellular biology · 1998Article
- In vitro genetic analysis of the RNA binding site of vigilin, a multi-KH-domain protein.Molecular and cellular biology · 1998Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The transcript of the Saccharomyces cerevisiae MER2 gene is spliced efficiently during meiosis but not during vegetative growth. Efficient splicing of the wild-type MER2 transcript requires the Mer1 protein, which is produced only in meiotic cells. Analysis of deletion and substitution mutations in the MER2 5' exon demonstrates that the unusually large size of this exon plays an important role in splicing regulation. The cis-acting sequences essential for Mer1-dependent splicing of MER2 RNA were determined by the analysis of MER2 deletion mutants and hybrid genes. The 80-base MER2 intron is sufficient for Mer1-dependent splicing in vivo, but sequences in the 5' exon enhance splicing efficiency. The Mer1 protein contains the KH motif found in some RNA-binding proteins, and RNA gel mobility shift assays demonstrate that Mer1 binds specifically to MER2 RNA. Both the transcript derived from the intronless MER2 gene and the transcript consisting only of the intron are able to bind to Mer1 in vitro, but neither has as high affinity for the protein as the intact substrate. RNase T1 footprinting indicates that the Mer1 protein contacts MER2 RNA at several points in the 5' exon and in the intron. Thus, Mer1 interacts directly with a regulatory element in MER2 RNA and promotes splicing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.