ArticleMolecular and cellular biology1995
CAT8, a new zinc cluster-encoding gene necessary for derepression of gluconeogenic enzymes in the yeast Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed, 177 citations in OpenAlex.
- Mitochondrial respiration modulates Hsf1 activation and the heat shock response.bioRxiv : the preprint server for biology · 2026Article
- Decoding yeast transcriptional regulation via a data-and mechanism-driven distributed large-scale network model.Synthetic and systems biotechnology · 2025Article
- Upregulation of the gluconeogenesis pathway was observed byFood science and biotechnology · 2025Article
- Article
- Yeast zinc cluster transcription factors involved in the switch from fermentation to respiration show interdependency for DNA binding revealing a novel type of DNA recognition.Nucleic acids research · 2024Article
- Article
- Regulation of Cat8 in energy metabolic balance and glucose tolerance in Saccharomyces cerevisiae.Applied microbiology and biotechnology · 2023Article
- The biological relevance of the FspTF transcription factor, homologous of Bqt4, inFrontiers in microbiology · 2023Article
- Cat8 Response to Nutritional Changes and Interaction With Ehrlich Pathway Related Factors.Frontiers in microbiology · 2022Article
- Modular and Molecular Optimization of a LOV (Light-Oxygen-Voltage)-Based Optogenetic Switch in Yeast.International journal of molecular sciences · 2021Article
- Two homologs of the Cat8 transcription factor are involved in the regulation of ethanol utilization in Komagataella phaffii.Current genetics · 2021Article
- Pbp1, the yeast ortholog of human Ataxin-2, functions in the cell growth on non-fermentable carbon sources.PloS one · 2021Article
- Deciphering eukaryotic gene-regulatory logic with 100 million random promoters.Nature biotechnology · 2020Article
- The E3 ubiquitin ligase Pib1 regulates effective gluconeogenic shutdown upon glucose availability.The Journal of biological chemistry · 2019Article
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- Alleviation of catabolite repression inBiotechnology for biofuels · 2019Article
- Conventional and emerging roles of the energy sensor Snf1/AMPK inMicrobial cell (Graz, Austria) · 2018Review
- Snf1/AMPK is involved in the mitotic spindle alignment in Saccharomyces cerevisiae.Scientific reports · 2018Article
- Reconstruction of metabolic module with improved promoter strength increases the productivity of 2-phenylethanol in Saccharomyces cerevisiae.Microbial cell factories · 2018Article
- Transcriptome analysis of Δmig1Δmig2 mutant reveals their roles in methanol catabolism, peroxisome biogenesis and autophagy in methylotrophic yeast Pichia pastoris.Genes & genomics · 2018Article
25 more citing papers are in PubMed but not listed here.
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The expression of gluconeogenic fructose-1,6-bisphosphatase (encoded by the FBP1 gene) depends on the carbon source. Analysis of the FBP1 promoter revealed two upstream activating elements, UAS1FBP1 and UAS2FBP1, which confer carbon source-dependent regulation on a heterologous reporter gene. On glucose media neither element was activated, whereas after transfer to ethanol a 100-fold derepression was observed. This gene activation depended on the previously identified derepression genes CAT1 (SNF1) (encoding a protein kinase) and CAT3 (SNF4) (probably encoding a subunit of Cat1p [Snf1p]). Screening for mutations specifically involved in UAS1FBP1 derepression revealed the new recessive derepression mutation cat8. The cat8 mutants also failed to derepress UAS2FBP1, and these mutants were unable to grow on nonfermentable carbon sources. The CAT8 gene encodes a zinc cluster protein related to Saccharomyces cerevisiae Gal4p. Deletion of CAT8 caused a defect in glucose derepression which affected all key gluconeogenic enzymes. Derepression of glucose-repressible invertase and maltase was still normally regulated. A CAT8-lacZ promoter fusion revealed that the CAT8 gene itself is repressed by Cat4p (Mig1p). These results suggest that gluconeogenic genes are derepressed upon binding of Cat8p, whose synthesis depends on the release of Cat4p (Mig1p) from the CAT8 promoter. However, gluconeogenic promoters are still glucose repressed in cat4 mutants, which indicates that in addition to its transcription, the Cat8p protein needs further activation. The observation that multicopy expression of CAT8 reverses the inability of cat1 and cat3 mutants to grow on ethanol indicates that Cat8p might be the substrate of the Cat1p/Cat3p protein kinase.
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