ArticleMolecular and cellular biology1995
Role of the Saccharomyces cerevisiae general regulatory factor CP1 in methionine biosynthetic gene transcription.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- Integration of metabolic reactions and gene regulation.Molecular biotechnology · 2011Review
- Combinatorial influence of environmental parameters on transcription factor activity.Bioinformatics (Oxford, England) · 2008Article
- Reconstructing dynamic regulatory maps.Molecular systems biology · 2007Article
- Roles for Gcn5p and Ada2p in transcription and nucleotide excision repair at the Saccharomyces cerevisiae MET16 gene.Nucleic acids research · 2006Article
- The histone fold domain of Cse4 is sufficient for CEN targeting and propagation of active centromeres in budding yeast.Eukaryotic cell · 2004Article
- Inhibition of 5' to 3' mRNA degradation under stress conditions in Saccharomyces cerevisiae: from GCN4 to MET16.RNA (New York, N.Y.) · 2004Article
- Cbf1p modulates chromatin structure, transcription and repair at the Saccharomyces cerevisiae MET16 locus.Nucleic acids research · 2004Article
- The Hsp70-Ydj1 molecular chaperone represses the activity of the heme activator protein Hap1 in the absence of heme.Molecular and cellular biology · 2001Article
- Transcriptional profiling shows that Gcn4p is a master regulator of gene expression during amino acid starvation in yeast.Molecular and cellular biology · 2001Article
- In vivo chromatin remodeling by yeast ISWI homologs Isw1p and Isw2p.Genes & development · 2001Article
- SURVEY AND SUMMARY: Saccharomyces cerevisiae basic helix-loop-helix proteins regulate diverse biological processes.Nucleic acids research · 2000Review
- Multiple transcriptional activation complexes tether the yeast activator Met4 to DNA.The EMBO journal · 1998Article
- Mutations synthetically lethal with cep1 target S. cerevisiae kinetochore components.Genetics · 1998Article
- Metabolism of sulfur amino acids in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 1997Review
- Yeast microarrays for genome wide parallel genetic and gene expression analysis.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Assembly of a bZIP-bHLH transcription activation complex: formation of the yeast Cbf1-Met4-Met28 complex is regulated through Met28 stimulation of Cbf1 DNA binding.The EMBO journal · 1997Article
- Dosage suppressors of a benomyl-dependent tubulin mutant: evidence for a link between microtubule stability and cellular metabolism.Genetics · 1996Article
- Interactions of the yeast centromere and promoter factor, Cpf1p, with the cytochrome c1 upstream region and functional implications on regulated gene expression.Nucleic acids research · 1996Article
- A heteromeric complex containing the centromere binding factor 1 and two basic leucine zipper factors, Met4 and Met28, mediates the transcription activation of yeast sulfur metabolism.The EMBO journal · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 3 countries.
Funding
Abstract
Saccharomyces cerevisiae general regulatory factor CP1 (encoded by the gene CEP1) is required for optimal chromosome segregation and methionine prototrophy. MET16-CYC1-lacZ reporter constructs were used to show that MET16 5'-flanking DNA contains a CP1-dependent upstream activation sequence (UAS). Activity of the UAS required an intact CP1-binding site, and the effects of cis-acting mutations on CP1 binding and UAS activity correlated. In most respects, MET16-CYC1-lacZ reporter gene expression mirrored that of chromosomal MET16; however, the endogenous gene was found to be activated in response to amino acid starvation (general control). The latter mechanism was both GCN4 and CP1 dependent. MET25 was also found to be activated by GCN4, albeit weakly. More importantly, MET25 transcription was strongly CP1 dependent in gcn4 backgrounds. The modulation of MET gene expression by GCN4 can explain discrepancies in the literature regarding CP1 dependence of MET gene transcription. Lastly, micrococcal nuclease digestion and indirect end labeling were used to analyze the chromatin structure of the MET16 locus in wild-type and cep1 cells. The results indicated that CP1 plays no major role in configuring chromatin structure in this region, although localized CP1-specific differences in nuclease sensitivity were detected.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.