ArticleInfection and immunity1995
Characterization of Pseudomonas aeruginosa-induced MDCK cell injury: glycosylation-defective host cells are resistant to bacterial killing.
Article in Infection and immunity, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
57 citing papers in PubMed, 105 citations in OpenAlex.
- Effects of influenza A infection on liberation of bacteria from biofilms and inflammatory response in anMicrobiology (Reading, England) · 2025Article
- Dual transcriptional analysis reveals adaptation of host and pathogen to intracellular survival of Pseudomonas aeruginosa associated with urinary tract infection.PLoS pathogens · 2021Article
- Contact lens-related corneal infection: Intrinsic resistance and its compromise.Progress in retinal and eye research · 2020Review
- Review
- Modulating Pathogenesis with Mobile-CRISPRi.Journal of bacteriology · 2019Article
- Effects of ε-Polylysine on Pseudomonas Aeruginosa and Aspergillus Fumigatus Biofilm In Vitro.Medical science monitor : international medical journal of experimental and clinical research · 2017Article
- Pathogenic Effects of Biofilm on Pseudomonas Aeruginosa Pulmonary Infection and Its Relationship to Cytokines.Medical science monitor : international medical journal of experimental and clinical research · 2016Article
- Pseudomonas aeruginosa Transmigrates at Epithelial Cell-Cell Junctions, Exploiting Sites of Cell Division and Senescent Cell Extrusion.PLoS pathogens · 2016Article
- The Role of ExoS in Dissemination of Pseudomonas aeruginosa during Pneumonia.PLoS pathogens · 2015Article
- Assessing Pseudomonas virulence using host cells.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Article
- Orientation of Pseudomonas aeruginosa ExsA monomers bound to promoter DNA and base-specific contacts with the P(exoT) promoter.Journal of bacteriology · 2012Article
- Subversion of mucosal barrier polarity by pseudomonas aeruginosa.Frontiers in microbiology · 2011Article
- ExsA recruits RNA polymerase to an extended -10 promoter by contacting region 4.2 of sigma-70.Journal of bacteriology · 2010Article
- Pseudomonas aeruginosa-mediated damage requires distinct receptors at the apical and basolateral surfaces of the polarized epithelium.Infection and immunity · 2010Article
- Mechanism of transcriptional activation by Pseudomonas aeruginosa ExsA.Journal of bacteriology · 2009Article
- Functional domains of ExsA, the transcriptional activator of the Pseudomonas aeruginosa type III secretion system.Journal of bacteriology · 2009Article
- Pathogenic phenotype and genotype of Pseudomonas aeruginosa isolates from spontaneous canine ocular infections.Investigative ophthalmology & visual science · 2009Article
- Cell wall glycans and soluble factors determine the interactions between the hyphae of Candida albicans and Pseudomonas aeruginosa.FEMS microbiology letters · 2008Article
- Pseudomonas aeruginosa induces localized immunosuppression during pneumonia.Infection and immunity · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
As a model for bacterium-induced epithelial cell injury, we have studied the interaction of Pseudomonas aeruginosa with polarized Madin-Darby canine kidney (MDCK) cells grown on filters. Following an initial period of bacterial adhesion, foci of injured host cells, which consisted of a central region of cell debris, surrounded by cells that were permeable and apparently necrotic, were formed. Host cell death was quantified by measuring the increased permeability of the monolayer to the macromolecular tracer [14C]inulin. Using this MDCK model system, we have identified bacterial and host cell factors necessary for the host cell damage. The ability of P. aeruginosa to cause MDCK cell damage was independent of elastase or exotoxin A production. In contrast, bacteria with a mutation in the regulatory locus exsA (which are deficient in exoenzyme S production) neither bound to nor caused host cell injury. MDCK cells with defects in cell surface glycosylation were resistant to cell injury, indicating that bacteria may require host cell glycolipids and/or glycoproteins as points of adhesion to cause subsequent host cell injury.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.