ArticleThe EMBO journal1995
Mitotic HeLa cells contain a CENP-E-associated minus end-directed microtubule motor.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 64 citations in OpenAlex.
- RZZ-Spindly and CENP-E form an integrated platform to recruit dynein to the kinetochore corona.The EMBO journal · 2023Article
- Genetic Primary Microcephalies: When Centrosome Dysfunction Dictates Brain and Body Size.Cells · 2023Review
- Leaving no-one behind: how CENP-E facilitates chromosome alignment.Essays in biochemistry · 2020Review
- Loss of c-Met disrupts gene expression program required for G2/M progression during liver regeneration in mice.PloS one · 2010Article
- CENP-E combines a slow, processive motor and a flexible coiled coil to produce an essential motile kinetochore tether.The Journal of cell biology · 2008Article
- A minimal CENP-A core is required for nucleation and maintenance of a functional human centromere.The EMBO journal · 2007Article
- Article
- Construction of neocentromere-based human minichromosomes by telomere-associated chromosomal truncation.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- CENP-meta, an essential kinetochore kinesin required for the maintenance of metaphase chromosome alignment in Drosophila.The Journal of cell biology · 2000Article
- Neocentromere formation in a stable ring 1p32-p36.1 chromosome.Journal of medical genetics · 1999Article
- Article
- Kinesin-like protein CENP-E is upregulated in rheumatoid synovial fibroblasts.Arthritis research · 1999Article
- Conservation of centromere protein in vertebrates.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1999Article
- Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1.The Journal of cell biology · 1998Article
- The vertebrate cell kinetochore and its roles during mitosis.Trends in cell biology · 1998Review
- CENP-E function at kinetochores is essential for chromosome alignment.The Journal of cell biology · 1997Article
- Centromere DNA dynamics: latent centromeres and neocentromere formation.American journal of human genetics · 1997Review
- The microtubule-dependent motor centromere-associated protein E (CENP-E) is an integral component of kinetochore corona fibers that link centromeres to spindle microtubules.The Journal of cell biology · 1997Article
- CENP-E is an essential kinetochore motor in maturing oocytes and is masked during mos-dependent, cell cycle arrest at metaphase II.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Kinetochores moving away from their associated pole do not exert a significant pushing force on the chromosome.The Journal of cell biology · 1996Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A minus end-directed microtubule motor activity from extracts of HeLa cells blocked at prometaphase/metaphase of mitosis with vinblastine has been partially purified and characterized. The motor activity was eliminated by immunodepletion of Centromere binding protein E (CENP-E). The CENP-E-associated motor activity, which was not detectable in interphase cells, moved microtubules at mean rates of 0.46 micron/s at 37 degrees C and 0.24 micron/s at 25 degrees C. The motor activity co-purified with CENP-E through several purification procedures. Motor activity was clearly not due to dynein or to kinesin. The microtubule gliding rates of the CENP-E-associated motor were different from those of dynein and kinesin. In addition, the pattern of nucleotide substrate utilization by the CENP-E-associated motor and the sensitivity to inhibitors were different from those of dynein and kinesin. The CENP-E-associated motor had an apparent native molecular weight of 874,000 Da and estimated dimensions of 2 nm x 80 nm. This is the first demonstration of motor activity associated with CENP-E, strongly supporting the hypothesis that CENP-E may act as a minus end-directed microtubule motor during mitosis.
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Registered trials
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