ArticleThe EMBO journal1995
The allele-specific synthetic lethality of prlA-prlG double mutants predicts interactive domains of SecY and SecE.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 73 citations in OpenAlex.
- Stepwise gating of the Sec61 protein-conducting channel by Sec63 and Sec62.Nature structural & molecular biology · 2021Article
- Genetic Analysis of Protein Translocation.The protein journal · 2019Review
- Article
- Dynamic action of the Sec machinery during initiation, protein translocation and termination.eLife · 2018Article
- Unlocking the Bacterial SecY Translocon.Structure (London, England : 1993) · 2016Article
- The bacterial Sec-translocase: structure and mechanism.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Review
- Structure of the SecY complex unlocked by a preprotein mimic.Cell reports · 2012Article
- Dynamics of SecY translocons with translocation-defective mutations.Structure (London, England : 1993) · 2010Article
- Oligomeric states of the SecA and SecYEG core components of the bacterial Sec translocon.Biochimica et biophysica acta · 2007Review
- The structure of the Sec complex and the problem of protein translocation.EMBO reports · 2006Review
- The plug domain of yeast Sec61p is important for efficient protein translocation, but is not essential for cell viability.Molecular biology of the cell · 2006Article
- Molecular dynamics studies of the archaeal translocon.Biophysical journal · 2006Article
- Modeling the effects of prl mutations on the Escherichia coli SecY complex.Journal of bacteriology · 2005Article
- Importance of transmembrane segments in Escherichia coli SecY.Molecular genetics and genomics : MGG · 2003Article
- Metabolic networks of microbial systems.Microbial cell factories · 2003Article
- Interfering mutations provide in vivo evidence that Escherichia coli SecE functions in multimeric states.Molecular genetics and genomics : MGG · 2003Article
- A SecE mutation that modulates SecY-SecE translocase assembly, identified as a specific suppressor of SecY defects.Journal of bacteriology · 2003Article
- Genetic evidence for parallel pathways of chaperone activity in the periplasm of Escherichia coli.Journal of bacteriology · 2001Article
- SecYEG assembles into a tetramer to form the active protein translocation channel.The EMBO journal · 2000Article
- The PrlA and PrlG phenotypes are caused by a loosened association among the translocase SecYEG subunits.The EMBO journal · 1999Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The secretion of proteins from the cytoplasm of Escherichia coli requires the interaction of two integral inner membrane components, SecY and SecE. We have devised a genetic approach to probe the molecular nature of the SecY-SecE interaction. Suppressor alleles of secY and secE, termed prlA and prlG, respectively, were analyzed in pair-wise combinations for synthetic phenotypes. From a total of 115 combinations, we found only seven pairs of alleles that exhibit a synthetic defect when present in combination with one another. The phenotypes observed are not the result of additive defects caused by the prl alleles, nor are they the consequence of multiple suppressors functioning within the same strain. In all cases, the synthetic defect is recessive to wild-type secY or secE provided in trans. The recessive nature argues for a defective interaction between the Prl suppressors. The extreme allele specificity and topological coincidence of the mutations represented by these seven pairs of alleles identify domains of interaction between SecY/PrlA and SecE/PrlG.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.