ArticlePlant molecular biology1995
Molecular characterization of a glyoxysomal citrate synthase that is synthesized as a precursor of higher molecular mass in pumpkin.
Article in Plant molecular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 72 citations in OpenAlex.
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- Ubiquitin-conjugating activity by PEX4 is required for efficient protein transport to peroxisomes in Arabidopsis thaliana.The Journal of biological chemistry · 2022Article
- Evolutionary Maintenance of the PTS2 Protein Import Pathway in the Stramenopile AlgaFrontiers in cell and developmental biology · 2020Article
- Melatonin promotes plant growth by maintaining integration and coordination between carbon and nitrogen metabolisms.Plant cell reports · 2019Article
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- Visualization of microbodies in Chlamydomonas reinhardtii.Journal of plant research · 2012Article
- Peroxisomal targeting signals in green algae.Protoplasma · 2009Article
- Arabidopsis peroxisomal citrate synthase is required for fatty acid respiration and seed germination.The Plant cell · 2005Article
- Presence of a bacterial-like citrate synthase gene in Tetrahymena thermophila: recent lateral gene transfers (LGT) or multiple gene losses subsequent to a single ancient LGT?Journal of molecular evolution · 2004Article
- Molecular characterization of an Arabidopsis acyl-coenzyme a synthetase localized on glyoxysomal membranes.Plant physiology · 2002Article
- Gene isolation and characterization of two acyl CoA oxidases from soybean with broad substrate specificities and enhanced expression in the growing seedling axis.Plant molecular biology · 2001Article
- ACX3, a novel medium-chain acyl-coenzyme A oxidase from Arabidopsis.Plant physiology · 2000Article
- The surprising complexity of peroxisome biogenesis.Plant molecular biology · 1998Review
- Characterization of a single soybean cDNA encoding cytosolic and glyoxysomal isozymes of aspartate aminotransferase.Plant molecular biology · 1998Article
- A light-independent developmental mechanism potentiates flavonoid gene expression in Arabidopsis seedlings.Plant molecular biology · 1998Article
- 2,4-Dichlorophenoxybutyric acid-resistant mutants of Arabidopsis have defects in glyoxysomal fatty acid beta-oxidation.The Plant cell · 1998Article
- The glyoxysomal and plastid molecular chaperones (70-kDa heat shock protein) of watermelon cotyledons are encoded by a single gene.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Protein transport into higher plant peroxisomes. In vitro import assay provides evidence for receptor involvement.Plant physiology · 1997Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA clone for glyoxysomal citrate synthase (gCS) was isolated from a lambda gt11 cDNA library prepared from etiolated pumpkin cotyledons. The cDNA of 1989 bp consisted of a 1548 bp open reading frame that encoded 516 amino acid residues. The deduced amino acid sequence of gCS did not have a typical peroxisomal targeting signal at its carboxyl terminal. A study of expression in vitro of the cDNA and an analysis of the amino-terminal sequence of the citrate synthase indicated that gCS is synthesized as a larger precursor that has a cleavable amino-terminal presequence of 43 amino acids. The predicted amino-terminal sequence of pumpkin gCS was highly homologous to those of other microbody enzymes, such as 3-ketoacyl-CoA thiolase of rat and malate dehydrogenase of watermelon that are also synthesized as precursors of higher molecular mass. Immunoblot analysis showed that the level of gCS protein increased markedly during germination and decreased rapidly during the light-induced transition of microbodies from glyoxysomes to leaf peroxisomes. By contrast, the level of mRNA for gCS reached a maximum earlier than that of the protein and declined even in darkness. The level of the mRNA was low during the microbody transition. These results indicate that the accumulation of the gCS protein does not correspond to that of the mRNA and that degradation of gCS is induced during the microbody transition, suggesting that post-transcriptional regulation plays an important role in the microbody transition.
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