ArticleApplied and environmental microbiology1995
Purification and characterization of alpha-L-arabinofuranosidase from Bacillus stearothermophilus T-6.
Article in Applied and environmental microbiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 104 citations in OpenAlex.
- Recombinant Production and Biochemical Characterization of Thermostable Arabinofuranosidase from Acidothermophilic Alicyclobacillus Acidocaldarius.The protein journal · 2023Article
- Structure and function characterization of the α-L-arabinofuranosidase from the white-rot fungus Trametes hirsuta.Applied microbiology and biotechnology · 2023Article
- A conserved enzyme of smut fungi facilitates cell-to-cell extension in the plant bundle sheath.Nature communications · 2022Article
- Purification and Biochemical Characterization of a Novel Thermostable Serine Protease from Geobacillus sp. GS53.Applied biochemistry and biotechnology · 2021Article
- The mechanism by which a distinguishing arabinofuranosidase can cope with internal di-substitutions in arabinoxylans.Biotechnology for biofuels · 2018Article
- Differential expression of α-L-arabinofuranosidases during maize (Zea mays L.) root elongation.Planta · 2015Article
- A novel bifunctional GH51 exo-α-l-arabinofuranosidase/endo-xylanase from Alicyclobacillus sp. A4 with significant biomass-degrading capacity.Biotechnology for biofuels · 2015Article
- Cloning, purification and preliminary crystallographic analysis of Ara127N, a GH127 β-L-arabinofuranosidase from Geobacillus stearothermophilus T6.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Crystallization and preliminary crystallographic analysis of Abp, a GH27 β-L-arabinopyranosidase from Geobacillus stearothermophilus.Acta crystallographica. Section F, Structural biology and crystallization communications · 2013Article
- A novel α-L-arabinofuranosidase of family 43 glycoside hydrolase (Ct43Araf) from Clostridium thermocellum.PloS one · 2013Article
- Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Structural insight into the ligand specificity of a thermostable family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum.The Biochemical journal · 2006Article
- Hyperthermophilic alpha-L: -arabinofuranosidase from Thermotoga maritima MSB8: molecular cloning, gene expression, and characterization of the recombinant protein.Extremophiles : life under extreme conditions · 2005Article
- Transcriptional regulation of genes encoding arabinan-degrading enzymes in Bacillus subtilis.Journal of bacteriology · 2004Article
- Purification and functional characterization of a novel alpha-L-arabinofuranosidase from Bifidobacterium longum B667.Applied and environmental microbiology · 2003Article
- Purification and partial characterization of alpha-L-arabinofuranosidase produced by Thermomonospora fusca.Folia microbiologica · 2003Article
- Characterization of two noncellulosomal subunits, ArfA and BgaA, from Clostridium cellulovorans that cooperate with the cellulosome in plant cell wall degradation.Journal of bacteriology · 2002Article
- Pseudomonas cellulosa expresses a single membrane-bound glycoside hydrolase family 51 arabinofuranosidase.The Biochemical journal · 2001Article
- Genetic and biochemical characterization of a highly thermostable alpha-L-arabinofuranosidase from Thermobacillus xylanilyticus.Applied and environmental microbiology · 2000Article
- The glucuronic acid utilization gene cluster from Bacillus stearothermophilus T-6.Journal of bacteriology · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus stearothermophilus T-6 produced an alpha-L-arabinofuranosidase when grown in the presence of L-arabinose, sugar beet arabinan, or oat spelt xylan. At the end of a fermentation, about 40% of the activity was extracellular, and enzyme activity in the cell-free supernatant could reach 25 U/ml. The enzymatic activity in the supernatant was concentrated against polyethylene glycol 20000, and the enzyme was purified eightfold by anion-exchange and hydrophobic interaction chromatographies. The molecular weight of T-6 alpha-L-arabinofuranosidase was 256,000, and it consisted of four identical subunits as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration. The native enzyme had a pI of 6.5 and was most active at 70 degrees C and at pH 5.5 to 6.0. Its thermostability at pH 7.0 was characterized by half-lives of 53, 15, and 1 h at 60, 65, and 70 degrees C, respectively. Kinetic experiments at 60 degrees C with p-nitrophenyl alpha-L-arabinofuranoside as a substrate gave a Vmax, a Km, and an activation energy of 749 U/mg, 0.42 mM, and 16.6 kcal/mol, (ca. 69.5 kJ/mol), respectively. The enzyme had no apparent requirement for cofactors, and its activity was strongly inhibited by 1 mM Hg2+. T-6 alpha-L-arabinofuranosidase released L-arabinose from arabinan and had low activity on oat spelt xylan. The enzyme acted cooperatively with T-6 xylanase in hydrolyzing oat spelt xylan, and L-arabinose, xylose, and xylobiose were detected as the end reaction products.(ABSTRACT TRUNCATED AT 250 WORDS)
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