ArticleThe American journal of pathology1995
Multiplex RT-PCR assay for the differential diagnosis of alveolar rhabdomyosarcoma and Ewing's sarcoma.
Article in The American journal of pathology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 103 citations in OpenAlex.
- Establishment of multiplex RT-PCR to detect fusion genes for the diagnosis of Ewing sarcoma.Diagnostic pathology · 2021Article
- Identification of the cross-strand chimeric RNAs generated by fusions of bi-directional transcripts.Nature communications · 2021Article
- Role of ancillary techniques in profiling unclassified laryngeal malignancies.Virchows Archiv : an international journal of pathology · 2018Review
- DNA Nanotweezers with Hydrolytic Activity for Enzyme-Free and Sensitive Detection of Fusion Gene via Logic Operation.Journal of analytical methods in chemistry · 2018Article
- Article
- Intergenically Spliced Chimeric RNAs in Cancer.Trends in cancer · 2016Review
- The comparative utility of fluorescence in situ hybridization and reverse transcription-polymerase chain reaction in the diagnosis of alveolar rhabdomyosarcoma.Virchows Archiv : an international journal of pathology · 2015Article
- Molecular strategies for detecting chromosomal translocations in soft tissue tumors (review).International journal of molecular medicine · 2014Review
- Molecular detection and targeting of EWSR1 fusion transcripts in soft tissue tumors.Medical oncology (Northwood, London, England) · 2013Review
- Soft tissue sarcomas with non-EWS translocations: molecular genetic features and pathologic and clinical correlations.Virchows Archiv : an international journal of pathology · 2010Review
- Rhabdomyosarcoma: molecular diagnostics of patients classified by morphology and immunohistochemistry with emphasis on bone marrow and purged peripheral blood progenitor cells involvement.Virchows Archiv : an international journal of pathology · 2006Article
- Article
- Detection of the PAX3-FKHR fusion gene in paediatric rhabdomyosarcoma: a reproducible predictor of outcome?British journal of cancer · 2001Article
- Review
- API2-MALT1 fusion transcripts involved in mucosa-associated lymphoid tissue lymphoma: multiplex RT-PCR detection using formalin-fixed paraffin-embedded specimens.The American journal of pathology · 2001Article
- Detection of micrometastasis by cytokeratin 20 RT-PCR is limited due to stable background transcription in granulocytes.British journal of cancer · 1999Article
- Methylation alterations of the MyoD1 upstream region are predictive of subclassification of human rhabdomyosarcomas.The American journal of pathology · 1998Article
- Article
- Is the EWS/FLI-1 fusion transcript specific for Ewing sarcoma and peripheral primitive neuroectodermal tumor? A report of four cases showing this transcript in a wider range of tumor types.The American journal of pathology · 1996Article
- Correlation between clinicopathological features and karyotype in lipomatous tumors. A report of 178 cases from the Chromosomes and Morphology (CHAMP) Collaborative Study Group.The American journal of pathology · 1996Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Cytogenetic analysis has defined specific translocations associated with two of the most common small round cell tumors of childhood, t(11;22) in Ewing's sarcoma and t(2;13) in alveolar rhabdomyosarcoma. We and others have previously demonstrated the diagnostic utility of a reverse transcriptase polymerase chain reaction (RT-PCR) assay for the detection of the t(11;22) encoded EWS/FLI-1 chimeric message in Ewing's sarcoma. More recently, we have cloned the t(2;13)(q35;q14) translocation and have shown that it results in the fusion of the PAX3 gene on chromosome 2 to FKHR, a novel member of the fork-head family of transcription factors on chromosome 13. To define the morphological spectrum of childhood sarcomas that express the t(2;13) encoded PAX3/FKHR chimeric message, we have performed RT-PCR analysis on samples from 44 primary pediatric sarcomas and 8 sarcoma cell lines. PAX3/FKHR chimeric messages were detected in 24 of 27 alveolar, 2 of 12 embryonal, and 0 of 1 pleomorphic rhabdomyosarcoma and in 1 of 2 ectomesenchymomas. In contrast, none of 8 Ewing's sarcomas or 2 undifferentiated sarcomas expressed this message. Chimeric transcripts were detected in all cases with cytogenetic evidence of the (2;13) translocation, and in each case the chimeric PAX3/FKHR message had the identical junction sequence, suggesting that genomic chromosome breaks were clustered in a single intron in both genes. By combining the PAX3/FKHR RT-PCR assay with primers for detection of the Ewing's sarcoma t(11;22) encoded EWS/FLI-1 chimeric transcript, we have developed a multiplex RT-PCR reaction that allows the rapid and accurate identification of either translocation in a biopsy sample.
Indexed as
Identifiers
7887445PMC1869175W2241382What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.