ArticleNucleic acids research1995
Interaction of the yeast splicing factor PRP8 with substrate RNA during both steps of splicing.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 65 citations in OpenAlex.
- Genetics and biochemistry remain essential in the structural era of the spliceosome.Methods (San Diego, Calif.) · 2017Review
- Role of Cwc24 in the First Catalytic Step of Splicing and Fidelity of 5' Splice Site Selection.Molecular and cellular biology · 2017Article
- Prp8 retinitis pigmentosa mutants cause defects in the transition between the catalytic steps of splicing.RNA (New York, N.Y.) · 2016Article
- Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes.PLoS genetics · 2015Article
- Rearrangements within human spliceosomes captured after exon ligation.RNA (New York, N.Y.) · 2013Article
- RNA helicases in splicing.RNA biology · 2013Review
- Structure and function of an RNase H domain at the heart of the spliceosome.The EMBO journal · 2008Article
- A conformational rearrangement in the spliceosome sets the stage for Prp22-dependent mRNA release.Molecular cell · 2008Article
- Inhibition of a spliceosome turnover pathway suppresses splicing defects.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- The network of protein-protein interactions within the human U4/U6.U5 tri-snRNP.RNA (New York, N.Y.) · 2006Article
- Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p.RNA (New York, N.Y.) · 2006Article
- Proximity of the U12 snRNA with both the 5' splice site and the branch point during early stages of spliceosome assembly.Molecular and cellular biology · 2005Article
- Prp8 protein: at the heart of the spliceosome.RNA (New York, N.Y.) · 2005Review
- Extended base pair complementarity between U1 snRNA and the 5' splice site does not inhibit splicing in higher eukaryotes, but rather increases 5' splice site recognition.Nucleic acids research · 2005Article
- Spatial organization of protein-RNA interactions in the branch site-3' splice site region during pre-mRNA splicing in yeast.Molecular and cellular biology · 2003Article
- Identification and characterization of Prp45p and Prp46p, essential pre-mRNA splicing factors.RNA (New York, N.Y.) · 2003Article
- Reversible inhibition of the second step of splicing suggests a possible role of zinc in the second step of splicing.Nucleic acids research · 2002Article
- Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs.Molecular and cellular biology · 2002Article
- Functional analyses of interacting factors involved in both pre-mRNA splicing and cell cycle progression in Saccharomyces cerevisiae.RNA (New York, N.Y.) · 2000Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PRP8 protein of Saccharomyces cerevisiae interacts directly with pre-mRNA in spliceosomes, shown previously by UV-crosslinking. To analyse at which steps of splicing and with which precursor-derived RNA species the interaction(s) take place, UV-crosslinking was combined with PRP8-specific immunoprecipitation and the coprecipitated RNA species were analysed. Specific precipitation of intron-exon 2 and excised intron species was observed. PRP8 protein could be UV-crosslinked to pre-mRNA in PRP2-depleted spliceosomes stalled before initiation of the splicing reaction. Thus, the interaction of PRP8 protein with substrate RNA is established prior to the first transesterification reaction, is maintained during both steps of splicing and continues with the excised intron after completion of the splicing reaction. RNase T1 treatment of spliceosomes revealed that substrate RNA fragments of the 5' splice site region and the branchpoint-3' splice site region could be coimmunoprecipitated with PRP8 specific antibodies, indicating that these are potential sites of interaction for PRP8 protein with substrate RNA. Protection of the branch-point-3' splice site region was detected only after step 1 of splicing. The results allow a first glimpse at the pattern of PRP8 protein-RNA interactions during splicing and provide a fundamental basis for future analysis of these interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.