ArticleJournal of clinical microbiology1994
Rapid identification of bacteria by PCR-single-strand conformation polymorphism.
Article in Journal of clinical microbiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 98 citations in OpenAlex.
- Plant Growth-Promoting Ability of MycorrhizalFrontiers in microbiology · 2022Article
- IL-1R and MyD88 Contribute to the Absence of a Bacterial Microbiome on the Healthy Murine Cornea.Frontiers in microbiology · 2018Article
- Capillary Electrophoresis Single-Strand Conformational Polymorphisms as a Method to Differentiate Algal Species.Journal of analytical methods in chemistry · 2015Article
- Aeromonas detection and characterization using genus-specific PCR and single-strand conformation polymorphism (SSCP).World journal of microbiology & biotechnology · 2012Article
- An efficient multistrategy DNA decontamination procedure of PCR reagents for hypersensitive PCR applications.PloS one · 2010Article
- Impact of free-living amoebae on presence of Parachlamydia acanthamoebae in the hospital environment and its survival in vitro without requirement for amoebae.Journal of clinical microbiology · 2010Article
- Evaluation of the alkaline wash/lysis procedure for the molecular diagnosis of a positive bacterial blood culture in clinical routine practice.Journal of clinical laboratory analysis · 2010Article
- Nucleotide sequencing and analysis of 16S rDNA and 16S-23S rDNA internal spacer region (ISR) of Taylorella equigenitalis, as an important pathogen for contagious equine metritis (CEM).Veterinary research communications · 2006Article
- Central line-related bacteraemia due to Tsukamurella tyrosinosolvens in a haematology patient.The Ulster medical journal · 2005Article
- A two-step species-specific 16S rRNA PCR assay for the detection of Taylorella equigenitalis in horses.Irish veterinary journal · 2005Article
- False-positive results and contamination in nucleic acid amplification assays: suggestions for a prevent and destroy strategy.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2004Review
- Methodologies for the characterization of microbes in industrial environments: a review.Journal of industrial microbiology & biotechnology · 2003Review
- Comparison of different decontamination methods for reagents to detect low concentrations of bacterial 16S DNA by real-time-PCR.Molecular biotechnology · 2002Article
- Characterization of Salmonella serovars by PCR-single-strand conformation polymorphism analysis.Journal of clinical microbiology · 2002Article
- Rapid detection of sepsis complicating acute necrotizing pancreatitis using polymerase chain reaction.World journal of gastroenterology · 2001Article
- Identification by 16S ribosomal RNA gene sequencing of an Enterobacteriaceae species from a bone marrow transplant recipient.Molecular pathology : MP · 2000Article
- Capillary electrophoresis-single-strand conformation polymorphism analysis for rapid identification of Pseudomonas aeruginosa and other gram-negative nonfermenting bacilli recovered from patients with cystic fibrosis.Journal of clinical microbiology · 1999Article
- Microbiological and clinical aspects of infection associated with Stenotrophomonas maltophilia.Clinical microbiology reviews · 1998Review
- Decontamination of polymerase chain reaction reagents for detection of low concentrations of 16S rRNA genes.Molecular biotechnology · 1997Article
- Species identification of Chlamydia isolates by analyzing restriction fragment length polymorphism of the 16S-23S rRNA spacer region.Journal of clinical microbiology · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new molecular biological approach for the identification of bacteria is described. This approach employs PCR of bacterial cell lysates with conserved primers located in the 16S rRNA sequence flanking a variable region, and analysis of the amplified product was based on the principle of single-strand conformation polymorphism (SSCP). The PCR product was denatured and separated on a nondenaturing polyacrylamide gel. SSCP patterns were detected by silver staining the nucleic acids. The mobility of the single-stranded DNA is sequence dependent and could be used to identify the unknown bacteria. Feasibility of the technique was demonstrated for a broad panel of gram-negative and gram-positive bacteria. We tested over 100 strains of bacteria representing 15 genera and 40 species. With the use of only two primer sets, P11P-P13P and ER10-ER11, we were capable to discriminate the tested species at the genus and species levels. Species-specific patterns were obtained for, e.g., Clostridium spp., Listeria spp., Pseudomonas spp., and Enterobacter spp. PCR-SSCP is a sensitive technique; e.g., the sensitivity obtained for Escherichia coli cells was 30 CFU. This technique is a simple and rapid method for the detection and identification of a wide spectrum of bacteria by whole-cell-based PCR amplification with the use of conserved primers and identification by nondenaturing gel electrophoresis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.