ArticleThe EMBO journal1995
Purification of the sequence-specific transcription factor CTCBF, involved in the control of human collagen IV genes: subunits with homology to Ku antigen.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 71 citations in OpenAlex.
- Identification of new DNA-associated proteins from Waddlia chondrophila.Scientific reports · 2019Article
- Purification and identification of positive regulators binding to a novel element in the c-Jun promoter.Biochemistry · 2008Article
- Bifunctional promoter of type IV collagen COL4A5 and COL4A6 genes regulates the expression of alpha5 and alpha6 chains in a distinct cell-specific fashion.The Biochemical journal · 2005Article
- KAP-2, a protein that binds to the H-box in a bean chalcone synthase promoter, is a novel plant transcription factor with sequence identity to the large subunit of human Ku autoantigen.Plant molecular biology · 2002Article
- The characterization and purification of a human transcription factor modulating the glutathione peroxidase gene in response to oxygen tension.Molecular and cellular biochemistry · 2002Article
- Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription.Molecular and cellular biology · 1999Article
- OBA/Ku86: DNA binding specificity and involvement in mammalian DNA replication.Molecular biology of the cell · 1999Article
- Purification and characterization of a cellular protein that binds to the downstream activation sequence of the strict late UL38 promoter of herpes simplex virus type 1.Journal of virology · 1998Article
- Interaction of Ku protein and DNA-dependent protein kinase catalytic subunit with nucleic acids.Nucleic acids research · 1998Review
- The 86-kD subunit of Ku autoantigen mediates homotypic and heterotypic adhesion of multiple myeloma cells.The Journal of clinical investigation · 1998Article
- Sp1-mediated transactivation of LamC1 promoter and coordinated expression of laminin-gamma1 and Sp1 in human hepatocellular carcinomas.The American journal of pathology · 1997Article
- KARP-1: a novel leucine zipper protein expressed from the Ku86 autoantigen locus is implicated in the control of DNA-dependent protein kinase activity.The EMBO journal · 1997Article
- Role of NRF-1 in bidirectional transcription of the human GPAT-AIRC purine biosynthesis locus.Nucleic acids research · 1997Article
- DNA looping by Ku and the DNA-dependent protein kinase.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Ku-related antigens are associated with transcriptionally active loci in Chironomus polytene chromosomes.Chromosoma · 1996Article
- Modulation of thermal induction of hsp70 expression by Ku autoantigen or its individual subunits.Molecular and cellular biology · 1996Article
- Hdf1, a yeast Ku-protein homologue, is involved in illegitimate recombination, but not in homologous recombination.Nucleic acids research · 1996Article
- Genetic characterization of transactivation of the human T-cell leukemia virus type 1 promoter: Binding of Tax to Tax-responsive element 1 is mediated by the cyclic AMP-responsive members of the CREB/ATF family of transcription factors.Molecular and cellular biology · 1996Article
- Expression of laminin gamma 1 cultured hepatocytes involves repeated CTC and GC elements in the LAMC1 promoter.The Biochemical journal · 1996Article
- Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The common promoter region of the human collagen type IV genes COL4A1 and COL4A2 comprises a C5TC7 sequence ('CTC box') which is specifically recognized by the recently identified transcription factor CTC box binding factor (CTCBF) involved in the control of divergent transcription of the two genes. This factor has now been purified by affinity chromatography on heparin-agarose and CTC-Sepharose. The CTCBF contains two subunits, CTC75 and CTC85, with molecular weights of 75 and 85 kDa, respectively. Sequence analysis of LysC-derived peptides of the two subunits revealed identity or close homology to p70 and p80 subunits of the human autoantigen Ku. The sequence-specific binding CTCBF represents a presumably tetrameric complex composed of two CTC75/85 heterodimers with an apparent molecular weight of 360-400 kDa. UV crosslinking experiments, the use of Ku-specific antibodies in gel retardation assays and immunoblotting proved that both subunits are involved in sequence-specific interaction with the CTC box motif. The tetrameric complex dissociates in a concentration-dependent manner to CTC75/85 heterodimers which now bind sequence independently to DNA. Three lines of evidence indicate that TATA binding protein (TBP) is additionally involved in the formation of CTCBF: (i) TBP can be detected in purified CTCBF; (ii) the addition of recombinant TBP stimulates formation of the CTCBF-DNA complex; and (iii) antibodies directed against TBP interfere strongly with the formation of the specific protein-DNA complex. The results presented support the idea that the subunits CTC75 and CTC85 (identical or homologous to p70 and p80 of the Ku antigen) are integral parts of CTCBF, and give a first indication of the importance of TBP in the formation of CTCBF.
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