ArticleThe EMBO journal1995
Nuclear translocation of a maternal CCAAT factor at the start of gastrulation activates Xenopus GATA-2 transcription.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 43 citations in OpenAlex.
- The role(s) of NF-Y in development and differentiation.Cell death and differentiation · 2025Review
- Control of zygotic genome activation in Xenopus.Current topics in developmental biology · 2021Review
- ILF-3 is a regulator of the neural plate border marker Zic1 in chick embryos.Developmental dynamics : an official publication of the American Association of Anatomists · 2012Article
- Alternative promoter and GATA5 transcripts in mouse.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- An Oct-1 binding site mediates activation of the gata2 promoter by BMP signaling.Nucleic acids research · 2005Article
- The LTR enhancer of ERV-9 human endogenous retrovirus is active in oocytes and progenitor cells in transgenic zebrafish and humans.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Exportin-5, a novel karyopherin, mediates nuclear export of double-stranded RNA binding proteins.The Journal of cell biology · 2002Article
- RNA-dependent cytoplasmic anchoring of a transcription factor subunit during Xenopus development.The EMBO journal · 2000Article
- Rescue of the embryonic lethal hematopoietic defect reveals a critical role for GATA-2 in urogenital development.The EMBO journal · 1998Article
- Xenopus NF-Y pre-sets chromatin to potentiate p300 and acetylation-responsive transcription from the Xenopus hsp70 promoter in vivo.The EMBO journal · 1998Article
- The maternal CCAAT box transcription factor which controls GATA-2 expression is novel and developmentally regulated and contains a double-stranded-RNA-binding subunit.Molecular and cellular biology · 1998Article
- Methods for the analysis of DNA-protein interactions.Molecular biotechnology · 1997Review
- Promoter analysis in living zebrafish embryos identifies a cis-acting motif required for neuronal expression of GATA-2.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Over-expression of GATA-6 in Xenopus embryos blocks differentiation of heart precursors.The EMBO journal · 1997Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The transcription factor GATA-2 is present in blood cell precursors and plays a pivotal role in the control of erythroid differentiation. In Xenopus embryos, low levels of GATA-2 mRNA are maternally derived, while the onset of zygotic GATA-2 expression coincides with commitment to haematopoietic lineages. However, its initial transcriptional activation is not restricted to the presumptive blood islands, but occurs throughout ventral and lateral regions, in all three germ layers. In order to determine how this expression pattern is controlled, we have isolated and characterized the Xenopus GATA-2 gene. We show that 1.65 kb of 5' flanking sequences are sufficient to direct both correct transcriptional initiation in oocytes and appropriate temporal and spatial gene expression in early embryos. The transgene is activated during gastrulation and by neurula stages in predominantly expressed in the ventral hemisphere. We demonstrate that a CCAAT element is necessary for gene activity in both systems and that extracts prepared from oocytes and embryos contain a factor which specifically recognizes this element. We also show that cytoplasmic localization inhibits the function of this CCAAT factor until the beginning of gastrulation, when the zygotic GATA-2 gene is activated. These observations extend our understanding of the mechanisms by which maternal factors control the temporal activation of transcription in early vertebrate embryos.
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