ArticleTransgenic research1995
Expression of genomic and cDNA transgenes after co-integration in transgenic mice.
Article in Transgenic research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 25 citations in OpenAlex.
- Hierarchy within the mammary STAT5-driven Wap super-enhancer.Nature genetics · 2016Article
- Epigenetic mechanisms affect mutant p53 transgene expression in WAP-mutp53 transgenic mice.Oncogene · 2005Article
- Making better transgenic models: conditional, temporal, and spatial approaches.Molecular biotechnology · 2005Review
- Secretion of recombinant human fibrinogen by the murine mammary gland.Transgenic research · 2004Article
- Phenotypic and genotypic stability of multiple lines of transgenic pigs expressing recombinant human protein C.Transgenic research · 1997Article
- Mammalian cDNA and prokaryotic reporter sequences silence adjacent transgenes in transgenic mice.Nucleic acids research · 1997Article
- Transgene rescue in the mammary gland is associated with transcription but does not require translation of BLG transgenes.Transgenic research · 1997Article
- Elements within the beta-lactoglobulin gene inhibit expression of human serum albumin cDNA and minigenes in transfected cells but rescue their expression in the mammary gland of transgenic mice.Nucleic acids research · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In general, genomic transgenes are expressed efficiently in mice, while their cDNA-based transgenes are frequently silent. Clark et al. (1992) have shown that silent cDNA transgenes under the control of the sheep beta-lactoglobulin promoter can be activated after co-injecting them with a genomic sheep beta-lactoglobulin transgene. We have tested the general utility of this concept using mouse whey acidic protein (WAP) transgenes. Here we show that WAP cDNA transgenes are virtually silent in transgenic mice. In contrast, WAP transgenes containing all introns are expressed in approximately 50% of the lines at levels ranging from 1% to more than 100% of the endogenous RNA (McKnight et al., 1992). When a WAP-genomic transgene was co-injected with a WAP-cDNA, basal activation of the cDNA was possible. However, the activity of the WAP-cDNA transgene did not exceed 1% of the WAP-genomic transgene. This suggests that a permissive integration site capable of supporting basal level transcription can be established, but further events are required for full activation of the transgene.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.