ArticleProceedings of the National Academy of Sciences of the United States of America1995
Segregation of cardiac and skeletal muscle-specific regulatory elements of the beta-myosin heavy chain gene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 36 citations in OpenAlex.
- Construction and Development of a Cardiac Tissue-Specific and Hypoxia-Inducible Expression Vector.Advanced pharmaceutical bulletin · 2018Article
- Sarcolipin deletion exacerbates soleus muscle atrophy and weakness in phospholamban overexpressing mice.PloS one · 2017Article
- Phospholamban overexpression in mice causes a centronuclear myopathy-like phenotype.Disease models & mechanisms · 2015Article
- Essential role of TEA domain transcription factors in the negative regulation of the MYH 7 gene by thyroid hormone and its receptors.PloS one · 2014Article
- Puralpha and Purbeta collaborate with Sp3 to negatively regulate beta-myosin heavy chain gene expression during skeletal muscle inactivity.Molecular and cellular biology · 2007Article
- Sp3 proteins negatively regulate beta myosin heavy chain gene expression during skeletal muscle inactivity.Molecular and cellular biology · 2004Article
- Induced mutant mouse lines that express lipoprotein lipase in cardiac muscle, but not in skeletal muscle and adipose tissue, have normal plasma triglyceride and high-density lipoprotein-cholesterol levels.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Organization of human and mouse skeletal myosin heavy chain gene clusters is highly conserved.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Multiple muscle-specific regulatory elements are associated with a DNase I hypersensitive site of the cardiac beta-myosin heavy-chain gene.The Biochemical journal · 1997Article
- E-box sites and a proximal regulatory region of the muscle creatine kinase gene differentially regulate expression in diverse skeletal muscles and cardiac muscle of transgenic mice.Molecular and cellular biology · 1996Article
- Position independent expression and developmental regulation is directed by the beta myosin heavy chain gene's 5' upstream region in transgenic mice.Nucleic acids research · 1995Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The beta-myosin heavy chain (beta-MyHC) gene is expressed in cardiac and slow skeletal muscles. To examine the regulatory sequences that are required for the gene's expression in the two compartments in vivo, we analyzed the expression pattern of a transgene consisting of the beta-MyHC gene 5' upstream region linked to the chloramphenicol acetyltransferase reporter gene. By using 5600 bp of 5' upstream region, the transgene was expressed at high levels in the slow skeletal muscles. Decreased levels of thyroid hormone led to the up-regulation of the transgene in both cardiac and skeletal muscles, mimicking the behavior of the endogenous beta-MyHC gene. After deleting the distal 5000 bp, the level of reporter gene expression was strongly reduced. However, decreased levels of thyroid hormone led to an 80-fold skeletal muscle-specific increase in transgene expression, even upon the ablation of a conserved cis-regulatory element termed MCAT, which under normal (euthyroid) conditions abolishes muscle-specific expression. In contrast, cardiac-specific induction was not detected with the deletion construct. These observations indicate that the cardiac and skeletal muscle regulatory elements can be functionally segregated on the beta-MyHC gene promoter.
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