ArticleThe Journal of cell biology1995
Protein phosphatase 1 regulates the cytoplasmic dynein-driven formation of endoplasmic reticulum networks in vitro.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 114 citations in OpenAlex.
- CDR2 is a dynein adaptor recruited by kinectin to regulate ER sheet organization.The Journal of cell biology · 2025Article
- The Motility of Mouse Spermatozoa Changes Differentially After 30-Minute Exposure Under Simulating Weightlessness and Hypergravity.International journal of molecular sciences · 2024Article
- Changes in cortical endoplasmic reticulum clusters in the fertilized mouse oocyte†.Biology of reproduction · 2022Article
- Sperm of Fruit FlyInternational journal of molecular sciences · 2022Article
- Intertwined and Finely Balanced: Endoplasmic Reticulum Morphology, Dynamics, Function, and Diseases.Cells · 2021Review
- Network organisation and the dynamics of tubules in the endoplasmic reticulum.Scientific reports · 2021Article
- A novel, dynein-independent mechanism focuses the endoplasmic reticulum around spindle poles in dividing Drosophila spermatocytes.Scientific reports · 2019Article
- A role for post-transcriptional control of endoplasmic reticulum dynamics and function in C. elegans germline stem cell maintenance.Development (Cambridge, England) · 2016Article
- Proteomic Analysis of Dynein-Interacting Proteins in Amyotrophic Lateral Sclerosis Synaptosomes Reveals Alterations in the RNA-Binding Protein Staufen1.Molecular & cellular proteomics : MCP · 2016Article
- The calcium-dependent ribonuclease XendoU promotes ER network formation through local RNA degradation.The Journal of cell biology · 2014Article
- Multiple mechanisms determine ER network morphology during the cell cycle in Xenopus egg extracts.The Journal of cell biology · 2013Article
- Analyses of dynein heavy chain mutations reveal complex interactions between dynein motor domains and cellular dynein functions.Genetics · 2012Article
- Unconventional functions of microtubule motors.Archives of biochemistry and biophysics · 2012Review
- Role of kinesin-1 and cytoplasmic dynein in endoplasmic reticulum movement in VERO cells.Journal of cell science · 2009Article
- Multivesicular bodies: co-ordinated progression to maturity.Current opinion in cell biology · 2008Review
- Dynein modifiers in C. elegans: light chains suppress conditional heavy chain mutants.PLoS genetics · 2007Article
- Cargo selection by specific kinesin light chain 1 isoforms.The EMBO journal · 2006Article
- APC is a component of an organizing template for cortical microtubule networks.Nature cell biology · 2005Article
- Phosphorylation controls CLIMP-63-mediated anchoring of the endoplasmic reticulum to microtubules.Molecular biology of the cell · 2005Article
- Chilling stability of microtubules in root-tip cells of cucumber.Plant cell reports · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interphase Xenopus egg extracts form extensive tubular membrane networks in vitro. These networks are identified here as endoplasmic reticulum by the presence of ER resident proteins, as shown by immunofluorescence, and by the presence of single ribosomes and polysomes, as shown by electron microscopy. The effect of phosphorylation on ER movement in interphase was tested using the phosphatase inhibitor, okadaic acid. Okadaic acid treatment resulted in an increase of up to 27-fold in the number of ER tubules moving and in the extent of ER networks formed compared to control extracts. This activation was blocked by the broad-specificity kinase inhibitor 6-dimethylaminopurine. Okadaic acid had no effect, however, on the direction of ER tubule movement, which occurred towards the minus end of microtubules, and was sensitive to low concentrations of vanadate. Inhibition of phosphatases also had no effect on the speed or duration of ER tubule extensions, and did not stimulate the activity of soluble cytoplasmic dynein. The sensitivity of ER movement to okadaic acid closely matched that of protein phosphatase 1. Although the amount of ER motility was greatly increased by inhibiting protein phosphatase 1 (PP1), the amount of cytoplasmic dynein associated with the membrane was not altered. The data support a model in which phosphorylation regulates ER movement by controlling the activity of cytoplasmic dynein bound to the ER membrane.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.