ArticleThe Journal of cell biology1995
Genetic deletion of ABP-120 alters the three-dimensional organization of actin filaments in Dictyostelium pseudopods.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 67 citations in OpenAlex.
- Review
- Mechanosensitive Adhesion Explains Stepping Motility in Amoeboid Cells.Biophysical journal · 2017Article
- Three-dimensional balance of cortical tension and axial contractility enables fast amoeboid migration.Biophysical journal · 2015Article
- Both contractile axial and lateral traction force dynamics drive amoeboid cell motility.The Journal of cell biology · 2014Article
- Cease-fire at the leading edge: new perspectives on actin filament branching, debranching, and cross-linking.Cytoskeleton (Hoboken, N.J.) · 2011Review
- The LRRK2-related Roco kinase Roco2 is regulated by Rab1A and controls the actin cytoskeleton.Molecular biology of the cell · 2011Article
- CP250, a novel acidic coiled-coil protein of the Dictyostelium centrosome, affects growth, chemotaxis, and the nuclear envelope.Molecular biology of the cell · 2009Article
- Actin binding domains direct actin-binding proteins to different cytoskeletal locations.BMC cell biology · 2008Article
- PC phosphorylation increases the ability of AFAP-110 to cross-link actin filaments.Molecular biology of the cell · 2002Article
- Relationship between Arp2/3 complex and the barbed ends of actin filaments at the leading edge of carcinoma cells after epidermal growth factor stimulation.The Journal of cell biology · 1999Article
- Capping protein terminates but does not initiate chemoattractant-induced actin assembly in Dictyostelium.The Journal of cell biology · 1997Article
- Myosin I overexpression impairs cell migration.The Journal of cell biology · 1997Article
- Interaction of a Dictyostelium member of the plastin/fimbrin family with actin filaments and actin-myosin complexes.Molecular biology of the cell · 1997Article
- Dictyostelium discoideum cells lacking the 34,000-dalton actin-binding protein can grow, locomote, and develop, but exhibit defects in regulation of cell structure and movement: a case of partial redundancy.The Journal of cell biology · 1996Article
- Re-expression of ABP-120 rescues cytoskeletal, motility, and phagocytosis defects of ABP-120- Dictyostelium mutants.Molecular biology of the cell · 1996Article
- Overlapping functions of myosin-I isoforms?The Journal of cell biology · 1996Review
- Dictyostelium mutants lacking multiple classic myosin I isoforms reveal combinations of shared and distinct functions.The Journal of cell biology · 1996Article
- Mechanical perturbation elicits a phenotypic difference between Dictyostelium wild-type cells and cytoskeletal mutants.Biophysical journal · 1996Article
- The Dictyostelium cytoskeleton.Experientia · 1995Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
This study extends the observations on the defects in pseudopod formation of ABP-120+ and ABP-120- cells by a detailed morphological and biochemical analysis of the actin based cytoskeleton. Both ABP-120+ and ABP-120- cells polymerize the same amount of F-actin in response to stimulation with cAMP. However, unlike ABP-120+ cells, ABP-120- cells do not incorporate actin into the Triton X-100-insoluble cytoskeleton at 30-50 s, the time when ABP-120 is incorporated into the cytoskeleton and when pseudopods are extended after cAMP stimulation in wild-type cells. By confocal and electron microscopy, pseudopods extended by ABP-120- cells are not as large or thick as those produced by ABP-120+ cells and in the electron microscope, an altered filament network is found in pseudopods of ABP-120- cells when compared to pseudopods of ABP-120+ cells. The actin filaments found in areas of pseudopods in ABP-120+ cells either before or after stimulation were long, straight, and arranged into space filling orthogonal networks. Protrusions of ABP-120- cells are less three-dimensional, denser, and filled with multiple foci of aggregated filaments consistent with collapse of the filament network due to the absence of ABP-120-mediated cross-linking activity. The different organization of actin filaments may account for the diminished size of protrusions observed in living and fixed ABP-120- cells compared to ABP-120+ cells and is consistent with the role of ABP-120 in regulating pseudopod extension through its cross-linking of actin filaments.
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