ArticleThe Journal of cell biology1995
Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 126 citations in OpenAlex.
- Lineage-Specific Diversification of Nucleoporin Nup98 Genes in Ciliates and Its Evolutionary Implications for the Nuclear Dualism.Journal of molecular evolution · 2026Article
- NovelCancers · 2023Article
- O-GlcNAc modification of nuclear pore complexes accelerates bidirectional transport.The Journal of cell biology · 2021Article
- Formation of Nup98-containing nuclear bodies in HeLa sublines is linked to genomic rearrangements affecting chromosome 11.Chromosoma · 2016Article
- O-GlcNAc-ylation in the Nuclear Pore Complex.Cellular and molecular bioengineering · 2016Article
- Intranuclear dynamics of the Nup107-160 complex.Molecular biology of the cell · 2015Article
- Analysis of the initiation of nuclear pore assembly by ectopically targeting nucleoporins to chromatin.Nucleus (Austin, Tex.) · 2015Article
- Nutrient-driven O-GlcNAc cycling - think globally but act locally.Journal of cell science · 2014Article
- Analysis of nuclear reconstitution, nuclear envelope assembly, and nuclear pore assembly using Xenopus in vitro assays.Methods in cell biology · 2014Article
- Monoclonal antibodies recognize gly-leu-phe-gly repeat of nucleoporin nup98 of tetrahymena, yeasts, and humans.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2013Article
- Systematic analysis of barrier-forming FG hydrogels from Xenopus nuclear pore complexes.The EMBO journal · 2013Article
- Domain topology of nucleoporin Nup98 within the nuclear pore complex.Journal of structural biology · 2012Article
- NUP98 gene fusions and hematopoietic malignancies: common themes and new biologic insights.Blood · 2011Review
- Amino-terminal enhancer of split (AES) interacts with the oncoprotein NUP98-HOXA9 and enhances its transforming ability.The Journal of biological chemistry · 2011Article
- Nup98 regulates bipolar spindle assembly through association with microtubules and opposition of MCAK.Molecular biology of the cell · 2011Article
- The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export.Molecular biology of the cell · 2010Article
- Inhibition of CRM1-mediated nuclear export of transcription factors by leukemogenic NUP98 fusion proteins.The Journal of biological chemistry · 2010Article
- Protein Tpr is required for establishing nuclear pore-associated zones of heterochromatin exclusion.The EMBO journal · 2010Article
- Effects of the NUP98-DDX10 oncogene on primary human CD34+ cells: role of a conserved helicase motif.Leukemia · 2010Article
- Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Xenopus egg extracts provide a powerful system for in vitro reconstitution of nuclei and analysis of nuclear transport. Such cell-free extracts contain three major N-acetylglucosaminylated proteins: p200, p97, and p60. Both p200 and p60 have been found to be components of the nuclear pore. Here, the role of p97 has been investigated. Xenopus p97 was isolated and antisera were raised and affinity purified. Immunolocalization experiments indicate that p97 is present in a punctate pattern on the nuclear envelope and also in the nuclear interior. Peptide sequence analysis reveals that p97 contains a GLFG motif which defines a family of yeast nuclear pore proteins, as well as a peptide that is identical at 11/15 amino acids to a specific member of the GLFG family, NUP116. An additional peptide is highly homologous to a second sequence found in NUP116 and other members of the yeast GLFG family. A monoclonal antibody to the GLFG domain cross-reacts with a major Xenopus protein of 97 kD and polyclonal antiserum to p97 recognizes the yeast GLFG nucleoporin family. The p97 antiserum was used to immunodeplete Xenopus egg cytosol and p97-deficient nuclei were reconstituted. The p97-depleted nuclei remained largely competent for nuclear protein import. However, in contrast to control nuclei, nuclei deficient in p97 fail to grow in size over time and do not replicate their chromosomal DNA. ssDNA replication in such extracts remains unaffected. Addition of the N-acetylglucosaminylated nuclear proteins of Xenopus or rat reverses these replication and growth defects. The possible role(s) of p97 in these nuclear functions is discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.