ArticleNucleic acids research1995
The importance of a single G in the hairpin loop of the iron responsive element (IRE) in ferritin mRNA for structure: an NMR spectroscopy study.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 56 citations in OpenAlex.
- Article
- The Iron Maiden. Cytosolic Aconitase/IRP1 Conformational Transition in the Regulation of Ferritin Translation and Iron Hemostasis.Biomolecules · 2021Article
- Review
- Iron-induced transferrin receptor-1 mRNA destabilization: A response to "Neither miR-7-5p nor miR-141-3p is a major mediator of iron-responsive transferrin receptor-1 mRNA degradation".RNA (New York, N.Y.) · 2019Article
- Catecholamine stress hormones regulate cellular iron homeostasis by a posttranscriptional mechanism mediated by iron regulatory protein: implication in energy homeostasis.The Journal of biological chemistry · 2015Article
- Structural and thermodynamic signatures that define pseudotriloop RNA hairpins.RNA (New York, N.Y.) · 2013Article
- Siderophore-mediated iron trafficking in humans is regulated by iron.Journal of molecular medicine (Berlin, Germany) · 2012Article
- Iron responsive mRNAs: a family of Fe2+ sensitive riboregulators.Accounts of chemical research · 2011Article
- Multiple determinants within iron-responsive elements dictate iron regulatory protein binding and regulatory hierarchy.RNA (New York, N.Y.) · 2010Article
- Direct Fe2+ sensing by iron-responsive messenger RNA:repressor complexes weakens binding.The Journal of biological chemistry · 2009Article
- Living with iron (and oxygen): questions and answers about iron homeostasis.Chemical reviews · 2009Review
- An iron responsive element-like stem-loop regulates alpha-hemoglobin-stabilizing protein mRNA.The Journal of biological chemistry · 2008Article
- The functional duality of iron regulatory protein 1.Current opinion in structural biology · 2008Review
- Molecular control of vertebrate iron homeostasis by iron regulatory proteins.Biochimica et biophysica acta · 2006Review
- Solution structure of the apical stem-loop of the human hepatitis B virus encapsidation signal.Nucleic acids research · 2006Article
- Structural polymorphism exhibited by a quasipalindrome present in the locus control region (LCR) of the human beta-globin gene cluster.Nucleic acids research · 2006Article
- A plant virus replication system to assay the formation of RNA pseudotriloop motifs in RNA-protein interactions.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- The Brome mosaic virus subgenomic promoter hairpin is structurally similar to the iron-responsive element and functionally equivalent to the minus-strand core promoter stem-loop C.RNA (New York, N.Y.) · 2002Article
- Molecular control of vertebrate iron metabolism: mRNA-based regulatory circuits operated by iron, nitric oxide, and oxidative stress.Proceedings of the National Academy of Sciences of the United States of America · 1996Review
- Role of RNA secondary structure of the iron-responsive element in translational regulation of ferritin synthesis.Nucleic acids research · 1995Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Noncoding sequences regulate the function of mRNA and DNA. In animal mRNAs, iron responsive elements (IREs) regulate the synthesis of proteins for iron storage, uptake and red cell heme formation. Folding of the IRE was indicated previously by reactivity with chemical and enzymatic probes. 1H- and 31P-NMR spectra now confirm the IRE folding; an atypical 31P-spectrum, differential accessibility of imino protons to solvents, multiple long-range NOEs and heat stable subdomains were observed. Biphasic hyperchromic transitions occurred (52 and 73 degrees C). A G-C base pair occurs in the hairpin loop (HL) (based on dimethylsulfate, RNAse T1 previously used, and changes in NMR imino proton resonances typical of G-C base pairs after G/A substitution). Mutation of the hairpin loop also decreased temperature stability and changed the 31P-NMR spectrum; regulation and protein (IRP) binding were previously shown to change. Alteration of IRE structure shown by NMR spectroscopy, occurred at temperatures used in studies of IRE function, explaining loss of IRP binding. The effect of the HL mutation on the IRE emphasizes the importance of HL structure in other mRNAs, viral RNAs (e.g. HIV-TAR), and ribozymes.
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