ArticleInfection and immunity1995
Antigenic and virulence properties of Pasteurella haemolytica leukotoxin mutants.
Article in Infection and immunity, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 48 citations in OpenAlex.
- Effect of a spore-based Mannheimia haemolytica vaccine on immune responses and respiratory microbiota in sheep.NPJ vaccines · 2026Article
- Comprehensive serotyping of Mannheimia haemolytica by a PCR system using the diversity of capsule biosynthesis genes.Scientific reports · 2025Article
- Mucosal Immunization with Spore-Based Vaccines againstVaccines · 2024Article
- Leukotoxin ofToxins · 2018Article
- Article
- Precise gene editing paves the way for derivation of Mannheimia haemolytica leukotoxin-resistant cattle.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Acylation Enhances, but Is Not Required for, the Cytotoxic Activity of Mannheimia haemolytica Leukotoxin in Bighorn Sheep.Infection and immunity · 2015Article
- Proximity-dependent inhibition of growth of Mannheimia haemolytica by Pasteurella multocida.Applied and environmental microbiology · 2012Article
- A multivalent Mannheimia-Bibersteinia vaccine protects bighorn sheep against Mannheimia haemolytica challenge.Clinical and vaccine immunology : CVI · 2011Article
- Bibersteinia trehalosi inhibits the growth of Mannheimia haemolytica by a proximity-dependent mechanism.Applied and environmental microbiology · 2010Article
- Intact signal peptide of CD18, the beta-subunit of beta2-integrins, renders ruminants susceptible to Mannheimia haemolytica leukotoxin.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Evolution of the leukotoxin promoter in genus Mannheimia.BMC evolutionary biology · 2009Article
- Analysis of gene order data supports vertical inheritance of the leukotoxin operon and genome rearrangements in the 5' flanking region in genus Mannheimia.BMC evolutionary biology · 2007Article
- Evidence for vertical inheritance and loss of the leukotoxin operon in genus Mannheimia.Journal of molecular evolution · 2007Article
- Mosaic structure and molecular evolution of the leukotoxin operon (lktCABD) in Mannheimia (Pasteurella) haemolytica, Mannheimia glucosida, and Pasteurella trehalosi.Journal of bacteriology · 2002Article
- Sequence diversity and molecular evolution of the leukotoxin (lktA) gene in bovine and ovine strains of Mannheimia (Pasteurella) haemolytica.Journal of bacteriology · 2001Article
- Inactivation of Pasteurella (Mannheimia) haemolytica leukotoxin causes partial attenuation of virulence in a calf challenge model.Infection and immunity · 2000Article
- Binding of Pasteurella haemolytica leukotoxin to bovine leukocytes.Infection and immunity · 1997Article
- Generation of targeted nonpolar gene insertions and operon fusions in Pasteurella haemolytica and creation of a strain that produces and secretes inactive leukotoxin.Infection and immunity · 1997Article
- Pasteurella haemolytica leukotoxin induces bovine leukocytes to undergo morphologic changes consistent with apoptosis in vitro.Infection and immunity · 1996Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antigenic properties of two mutants of Pasteurella haemolytica, strains 59B0071 and 59B0072, that do not produce detectable leukotoxin were investigated. Western blot (immunoblot) analysis with a number of polyclonal sera from animals recovering from pasteurellosis revealed that both mutants secreted a variety of antigens that were also present in cultures of several wild-type strains. These antigens ranged from about 100 to 15 kDa. Mutant strain 59B0071 was found to be totally deficient in leukotoxin, as judged not only by Western blotting but also by cytotoxicity assays with bovine lymphoma (BL-3) cells or bovine polymorphonuclear cells as targets. The mutant strain 59B0071 had normal levels of a secreted sialylglycoprotease, however. When strains were tested for virulence in goat and cattle challenge experiments, a reduction in mortality and lung lesions was observed with the mutant 59B0071 in comparison with results obtained with wild-type strains. These results are consistent with an important role for leukotoxin in P. haemolytica virulence and suggest that leukotoxin-negative mutants may be useful tools in the investigation of other virulence properties involved in P. haemolytica infections.
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